Abstract
This study was carried out to assess the potential use and applicability of micrografting technique for developing in vitro grafted plantlets. Microshoots of kiwifruit ‘Miliang-1’ and ‘Hongyang’ were used as rootstock and scion. Accomplishment of in vitro grafting has been examined by varying numerous factors, the physiological state of the rootstock and scion, the pH value of the medium, the concentration of sucrose, the type of medium and the PGRs. The results showed that the best conditions for in vitro grafting of kiwifruit were that the rootstock is not rooted and that both rootstock and scion were free of leaves. The best medium formula suitable for kiwifruit micrografting was 1/2 MS solid medium supplemented with 0.5 mg L−1 GA3, 1.0 mg L−1 IBA, 40 g L−1 sucrose and 7 g L−1 agar, pH 6.0. The perlite: peat soil: vermiculite volume ratio at 1:2:1 was the most suitable stroma formula for transplanting micrografted seedlings.
Key message
The results showed that the best conditions for in vitro grafting of kiwifruit were that the rootstock is not rooted and that both rootstock and scion were free of leaves. The best medium formula suitable for kiwifruit micrografting was 1/2 MS solid medium supplemented with 0.5 mg L−1 GA3, 1.0mg L−1 IBA, 40 g L−1 sucrose, and 7 g L−1 agar, pH 6.0. The perlite: peat soil: vermiculite volume ratio at 1:2:1 was the most suitable stroma formula for transplanting micrografted seedlings.
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Acknowledgements
The work was supported by grants from the Science and Technology Plan Project of Sichuan Province (Grant No. 2021YFN0085) and the Double Support Project of Discipline Construction of Sichuan Agricultural University (03573134).
Funding
Funding provided by Science and Technology Plan Project of Sichuan Province (2021YFN0085). Double Support Project of Discipline Construction of Sichuan Agricultural University (03573134).
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WY: Writing—original draft, Visualization; LK: Investigation, Methodology, Data curation; DL: Investigation, Validation; BZ: Methodology, Validation; HT: Software, Methodology; XZ: Investigation, Methodology; YL: Formal analysis; YZ: Investigation; QC: Software; YW: Visualization; ML: Investigation; WH: Investigation; YL: Visualization; XW: Visualization; HT: Project administration; YZ: Writing—review & editing, Conceptualization, Funding acquisition.
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Communicated by Sergio J. Ochatt.
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Yao, W., Kong, L., Lei, D. et al. Establishment of an efficient method for kiwifruit in vitro micrografting. Plant Cell Tiss Organ Cult 152, 427–436 (2023). https://doi.org/10.1007/s11240-022-02416-z
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DOI: https://doi.org/10.1007/s11240-022-02416-z