Skip to main content
SpringerLink
Log in

Induction of tetraploid via somatic embryos treated with colchicine in Hevea brasiliensis (Willd. ex A.Juss.) Müll. Arg

  • Research Note
  • Published:
Plant Cell, Tissue and Organ Culture (PCTOC) Aims and scope Submit manuscript

Abstract

Polyploidy is a potential way to breed new varieties with high yield and stress resistance in plants. In this study, we provided an efficient method for induction of tetraploid of Hevea brasiliensis by immerse somatic embryos in the liquid plant induction medium containing colchicine. The optimum concentration of colchicine is 5 mg/ml, and the optimum immersion time is 24–48 h. The highest average induction rate of tetraploid is 27.12% in Reyan73397 with 5 mg/ml colchicine for 48 h, and 38.89% in Reyan87662 with 5 mg/ml colchicine for 24 h. Finally, 82 tetraploids of Reyan73397 and 14 tetraploids of Reyan87662 were obtained. Compared to diploids, tetraploids exhibited less and lighter leaf veins, daker and thicker leaves, larger stomatal size and smaller stomatal density. These tetraploids have the potential to be used as parents to produce new triploid germplasms in Hevea brasiliensis breeding programs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  • Ao SC, He LG, Xiao GX, Chen JB, He CG (1998) Selection of rubber cold fast and high-yield strains, Yunyan77-2 and Yunyan 77 – 2. J Yunnan Trop Crops Sci Technol 21(2):3–8 (In Chinese with English abstract)

    Google Scholar 

  • del Pozo JC, Ramirez-Parra E (2014) Deciphering the molecular bases for drought tolerance in Arabidopsis autotetraploids. Plant Cell & Environ 37:2722–2737. https://doi.org/10.1111/pce.12344

    Article  CAS  Google Scholar 

  • Dhawan OP, Lavania UC (1996) Enhancing the productivity of secondary metabolites via induced polyploidy: a review. Euphytica 87:81–89. https://doi.org/10.1007/BF00021879

    Article  CAS  Google Scholar 

  • Eng WH, Ho WS (2019) Polyploidization using colchicine in horticultural plants: A review. Sci Hort 246:604–617. https://doi.org/10.1016/j.scienta.2018.11.010

    Article  CAS  Google Scholar 

  • Fernando SC, Goodger JQD, Chew BL, Cohen TJ, Woodrow IE (2019) Induction and characterisation of tetraploidy in Eucalyptus polybractea R.T. Baker. Industrial Crops & Products 140:111633. https://doi.org/10.1016/j.indcrop.2019.111633

    Article  CAS  Google Scholar 

  • Gao XS, Huang HS, Zhang XF, Wu CT, Wang XJ, Huang SB, Li WG (2012) The adaptability of 5 clones by independent selection in Hevea brasiliensis. Chin J Trop Crops 33(11):1921–1925 (In Chinese with English abstract)

  • Hamill DS, Smith KM, Dodd AW (1992) In vitro induction of banana autotetraploids by colchicine treatment of micropropagated diploids. Aust J Bot 40:887–896

  • Huang HS, Liang MH, Wu YT, Li DS, He JW (1994) Selection and breeding of a moderate scale clone SCATC 7-33-97. Chin J Tropical Crops 15(2):1–6 (In Chinese with English abstract)

  • Huang YJ, Qiu DB, Wang ZK, Xie SW (2003) Studies on morphological characters of somatic polyploids of Hevea brasiliensis. Chin J Trop crops 24(1):8–12 (In Chinese with English abstract)

    CAS  Google Scholar 

  • Li HB, Zhou TY, Ning LY, Li GH (2009) Cytological identification and breeding course of Hevea Yunyan77-2 and Yunyan77-4. J Trop Subtrop Bot 17(6):602–605 (In Chinese with English abstract)

    Google Scholar 

  • Ling XB, Chen GS (1988) Identification of artificial polyploids in Hevea brasiliensis by using bronze-coloured tender leaves and the technique of variants separation. Chin J Trop crops 9(2):11–16 (In Chinese with English abstract)

    Google Scholar 

  • Ling XB, Chen GS, Yang TY (1988) A method to increase polyploid induction rate in Hevea brasiliensis. Chin J Trop crops 9(2):1–9 (In Chinese with English abstract)

    Google Scholar 

  • Lu YL, Feng XY, Lu YY, Li WL (1980) Study on inducing polyploid plants of Hevea brasiliensis. Hereditas 2(6):23–26 (In Chinese)

    Google Scholar 

  • Luiz OT, Mendes AJ, Mendes T (1963) Colchicine induced polyploidy of Hevea rubber plants. Bragantia 22:383–392

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15(3):473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

    Article  CAS  Google Scholar 

  • Pootakham W, Sonthirod C, Naktang C, Ruang-Areerate P, Yoocha T, Sangsrakru D, Therawattanasuk K, Rattanawong R, Lekawipat N, Tangphatsornruang S (2017) De novo hybrid assembly of the rubber tree genome reveals evidence of paleotetraploidy in Hevea species. Sci Rep 7:41457. https://doi.org/10.1038/srep41457

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • van Beilen JB, Poirier Y (2007) Establishment of new crops for the production of natural rubber. Trends Biotechnol 25(11):522–529. https://doi.org/10.1016/j.tibtech.2007.08.009

    Article  CAS  PubMed  Google Scholar 

  • van de Peer Y, Ashman T, Soltis PS, Soltis DE (2021) Polyploidy: an evolutionary and ecological force in stressful times. Plant Cell 33:11–26. https://doi.org/10.1093/plcell/koaa015

    Article  PubMed  Google Scholar 

  • Wang LJ, Gao X, Jia GX (2021) Stomata and ROS changes during Botrytis elliptica infection in diploid and tetraploid Lilium rosthornii Diels. Plant Physiol Biochem 167:366–375. https://doi.org/10.1016/j.plaphy.2021.08.008

    Article  CAS  PubMed  Google Scholar 

  • Wu J, Sang YR, Zhou Q, Zhang PD (2020) Colchicine in vitro tetraploid induction of Populus hopeiensis from leaf blades. Plant Cell Tissue Organ Cult 141:339–349. https://doi.org/10.1007/s11240-020-01790-w

    Article  CAS  Google Scholar 

  • Xu CP, Huang Z, Liao T, Li Y, Kang XY (2016) In vitro tetraploid plants regeneration from leaf explants of multiple genotypes in Populus. Plant Cell Tissue Organ Cult 125:1–9. https://doi.org/10.1007/s11240-015-0922-0

    Article  CAS  Google Scholar 

  • Zeng X, Zhang S, Zhang Y, Zheng X, Chen H, Liu B, Zheng K (1997) Inducing of pure polyploid clone of Hevea by culturing somatic cell in vitro. Chin J Trop Crops 18(2):15–20 (In Chinese with English abstract)

    Google Scholar 

  • Zhang Y, Wang X, Gao X, Cai H, Huang H, Li W (2018) Induction of Anther Callus and Somatic Embryogenesis of Different Rubber Tree (Hevea brasiliensis Muell.Arg.) Clones. Chin Agric Sci Bull 34(3):70–75 (In Chinese with English abstract)

    Google Scholar 

  • Zhang YY, Zhang XF, Huang X, Zhang XY, Yao PQ, Li WG (2019) Low temperature increases the frequency of 2n female gametes in the diploid rubber tree (Hevea brasiliensis (Willd. ex A.Juss.) Müll. Arg.). Tree Genetcis & Genomes 15:23. https://doi.org/10.1007/s11295-019-1330-0

    Article  CAS  Google Scholar 

  • Zhou ZY, Zhan SR, Chen CQ, Guo QY, Liu JX (1989) Stomatal characteristics of polyploid plants of Hevea brasiliensis. Chin J Trop Agric 9(4):4–8 (In Chinese)

    Google Scholar 

  • Zhou HW, Zeng WD, Yan HB (2017) In vitro induction of tetraploids in cassava variety ‘Xinxuan 048’ using colchicine. Plant Cell Tissue Organ Cult 28:723–729. https://doi.org/10.1007/s11240-016-1141-z

    Article  CAS  Google Scholar 

  • Yildiz M (2013) Plant responses at different ploidy levels. In: Silva, M. (Ed.), Current Progress in Biological Research. Intech, pp. 363–385

Download references

Acknowledgements

This work was supported by the National Key Research and Development Program of China (2019YFD1001105) and the China Agriculture Research System (CARS-33-YZ2).

Author information

Authors and Affiliations

  1. Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, 571101, Haikou, Hainan, China

    Yuanyuan Zhang, Xiangjun Wang, Xiao Huang & Weiguo Li

  2. State Centre for Rubber Breeding, 571101, Haikou, Hainan, China

    Yuanyuan Zhang, Xiangjun Wang, Xiao Huang & Weiguo Li

  3. Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs, 571101, Haikou, Hainan, China

    Yuanyuan Zhang, Xiangjun Wang, Xiao Huang & Weiguo Li

Authors
  1. Yuanyuan Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiangjun Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiao Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Weiguo Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

YZ and WL conceived the projects. YZ and XW designed and executed the experiments. XH contributed materials/analysis tools. YZ performed data analysis and wrote the manuscript. All authors have read and approved the manuscript.

Corresponding author

Correspondence to Weiguo Li.

Ethics declarations

Conflict of interest

The authors declared that they have no potential conflict of interest.

Additional information

Communicated by Mohammad Reza Abdollahi.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, Y., Wang, X., Huang, X. et al. Induction of tetraploid via somatic embryos treated with colchicine in Hevea brasiliensis (Willd. ex A.Juss.) Müll. Arg. Plant Cell Tiss Organ Cult 150, 487–491 (2022). https://doi.org/10.1007/s11240-022-02281-w

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-022-02281-w

Keywords

Search

Navigation