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Silicon dioxide and silver nanoparticles elicit antimicrobial secondary metabolites while enhancing growth and multiplication of Lavandula officinalis in-vitro plantlets

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Abstract

Application of nanoparticles in plant tissue culture have been reported to improve growth and multiplication as well as alter compositions of bioactive compounds of different plant species. Lavender (Lavandula officinalis) is a medicinal plant, which produces essential oil and plant extracts that are rich in beta-linalool, cineol, camphor and many other bioactive compounds. Collectively, these compounds have contributed to its pain relieving, antimicrobial and wound healing activities. In this study, silicon dioxides nanoparticles or silver nanoparticles were incorporated into the growth media for Lavandula officinalis to investigate the effects of these nanomaterials on the growth, multiplication, photosynthetic pigments content, ultrastructure morphology, and genetic variation of the plantlets. In addition, the possible changes of phytochemicals composition and antimicrobial activities of the ethanolic extracts prepared from the plantlets were investigated. The incorporation of 50 mg/L Si-NPs highly enhanced multiplication of the explants, while 20 mg/L Ag-NPs resulted in small enhancement effects in multiplication and growth of the plantlets. The presence of nanoparticles in the growth media has altered the phytochemical compositions of the ethanolic extracts prepared from the L. officinalis plantlets, and these compositions differences have resulted in the differential antimicrobial activities of these extracts against Escherichia coli, methicillin resistance Staphylococcus aureus and Streptococcus pneumoniae. For E. coli and S. aureus, extract from plantlets grown in the presence of 100 mg/L Si-NPs exhibited the highest antimicrobial activity, while for S. pneumoniae, highest activity was observed with extract from plantlets grown without nanoparticles.

Key Message

Silicon dioxides or silver nanoparticles induces soma-clonal variation in lavender plantlets, while enhancing their growth and multiplications, and altered the chemical and biological activities of the plant extracts.

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Data availability

Data sharing not applicable to this article as no datasets were generated during the current study.

Notes

  1. nanoparticles.

  2. silver nanoparticles.

  3. silicon dioxide or silica nanoparticles.

  4. Gas chromatography-mass spectrometry.

  5. Inter Simple Sequence Repeats-Polymerase Chain Reaction.

  6. deoxyribonucleotides triphosphate.

  7. Colony forming unit per milliliter.

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Acknowledgements

The authors would like to thank Professor Mohamed El Hamahmy, Department of Botany, Faculty of Agriculture, Suez Canal University for analyzing the transmission and scanning electron micrographic images. Assistance from Miss Noorah Albotnoor in preparing and maintaining the lavender in-vitro plantlets is highly appreciated. The authors extend their appreciation to the Deanship of Scientific Research, King Faisal University for funding this research work through the project number “NA000143”.

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Authors and Affiliations

  1. Department of Biological Sciences, College of Science, King Faisal University, Al Hofuf, Saudi Arabia

    Salah Khattab, Fadia El Sherif, Munirah AlDayel, Yun-Kiam Yap & Hairul Islam Mohamed Ibrahim

  2. Department of Clinical Science, Central Lab, College of Veterinary Medicine, King Faisal University, Al Hofuf, Saudi Arabia

    Ahmed Meligy

  3. Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt

    Salah Khattab & Fadia El Sherif

  4. Department of Physiology, Agricultural Research Center (ARC), Giza, Egypt

    Ahmed Meligy

Authors
  1. Salah Khattab
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  2. Fadia El Sherif
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  3. Munirah AlDayel
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  4. Yun-Kiam Yap
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  5. Ahmed Meligy
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  6. Hairul Islam Mohamed Ibrahim
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Contributions

FS, YY, and SK conceived and designed the experiments. All authors (FS, MD, YY, AM, HI, and SK) performed the experiments. FS, YY, MD analyzed the data. FS and YY wrote the manuscript. All authors reviewed the manuscript.

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Correspondence to Yun-Kiam Yap.

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Communicated by K. X. Tang.

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Khattab, S., El Sherif, F., AlDayel, M. et al. Silicon dioxide and silver nanoparticles elicit antimicrobial secondary metabolites while enhancing growth and multiplication of Lavandula officinalis in-vitro plantlets. Plant Cell Tiss Organ Cult 149, 411–421 (2022). https://doi.org/10.1007/s11240-021-02224-x

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  • DOI: https://doi.org/10.1007/s11240-021-02224-x

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