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Advances in potato functional genomics: implications for crop improvement

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Abstract

Potato is one of the most important crops grown worldwide. The potato genome sequencing consortium has identified large numbers of genes having unknown functionality. To systematically assign functions to all predicted genes in its genome and their specific applications in potato improvement, different techniques have been developed over the years to generate mutants and analyze phenotypic variations among existing varieties. The generation of mutants could result in elucidating the function of genes and help in developing superior crop cultivars, thereby improving potato quality for future feed. In this review article, we have summarized various loss of function and gain of function genetic tools, which could be used for modifying or designing new strategies for the molecular engineering of potatoes. Moreover, the advantages and limitations of these tools suitable for mining genomic data have been discussed. This comprehensive summary could lay the foundation for the genetic improvement of potatoes towards food and nutritional security.

Key message

Functional genomics provides a better understanding of deciphering new experimental opportunities for developing nutritionally rich potato varieties in combating hidden hunger and developing new breeding programs for its improvement.

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Acknowledgements

The authors are thankful to the Central Potato Research Institute, Shimla for providing the necessary research facilities.

Funding

Science and Engineering Research Board, Department of Science & Technology (DST), Government of India, has funded the project in the form of a National-Post Doctoral Fellowship to Neha Sharma (PDF/2017/000131).

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Authors and Affiliations

  1. ICAR-Central Potato Research Institute, Shimla, Himachal Pradesh, 171001, India

    Neha Sharma, Sundaresha Siddappa, Kajal Thakur, Neha Salaria, Salej Sood & Vinay Bhardwaj

  2. ICAR-National Bureau of Plant Genetic Resources Regional Station, Shimla, Himachal Pradesh, 171004, India

    Nikhil Malhotra

Authors
  1. Neha Sharma
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  2. Sundaresha Siddappa
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  3. Nikhil Malhotra
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  4. Kajal Thakur
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  5. Neha Salaria
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  6. Salej Sood
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  7. Vinay Bhardwaj
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Contributions

NSh, SS conceived and conceptualized the article. VIGS, SIGS, TILLING, Activation tagging were reviewed by NSh and NM. RNAi was reviewed by SS, KT and NS. Mutagenesis and Genome editing techniques were reviewed by NSh, SS and VB. Final compilation and editing were done by NSh and SS. All authors read and approved the manuscript.

Corresponding author

Correspondence to Neha Sharma.

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The authors have no relevant financial or non-financial interests to disclose.

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Sharma, N., Siddappa, S., Malhotra, N. et al. Advances in potato functional genomics: implications for crop improvement. Plant Cell Tiss Organ Cult 148, 447–464 (2022). https://doi.org/10.1007/s11240-021-02221-0

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