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Mungbean VrCOL1 regulates flowering time under short-day conditions in Arabidopsis

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Abstract

CONSTANS-LIKE (COL) genes are important factors that play an important role in regulating photoperiod-dependent flowering time, and multiple COL genes have been identified in plants. However, knowledge of the COLs involved in photoperiod-dependent flowering time regulation is limited in mungbean (Vigna radiata). In this study, we characterized the function of VrCOL1 in regulating flowering time. VrCOL1 shares strong homology to the well-studied Arabidopsis AtCO and soybean GmCOL1a and GmCOL1b. VrCOL1/GmCOL1a and VrCOL1/GmCOL1b were demonstrated as two syntelogs, respectively. Subcellular localization analysis revealed that VrCOL1 is located in the nucleus. The expression of VrCOL1 increased under short-day (SD) conditions during growth phases, and the expression of VrCOL1 decreased under long-day (LD) conditions during growth phases. Overexpression of VrCOL1 accelerated flowering in Arabidopsis thaliana under SD conditions by activating the expression of FLOWERING LOCUS T (AtFT) and TWIN SISTER OF FT (AtTSF), whereas VrCOL1 had no effect on flowering time under LD conditions, indicating that VrCOL1 is involved in flowering time regulation under a photoperiod-dependent pathway. The results of our study provide new insights into the molecular mechanism of flowering time regulation in mungbean.

Key message

VrCOL1, an ortholog of AtCO in mungbean, accelerates flowering in Arabidopsis under short-day conditions by regulating the expression of AtFT and AtTSF.

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All authors have ensured that all data, and materials support the published claims and comply with field standards.

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Funding

This research was supported by the National Natural Science Foundation of China (Grant 31971898, 32171979), and the Qingdao Agricultural University Scientific Research Foundation (Grant 6631119010).

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Author notes

  1. Qianqian Zhang and Jing Dong have contributed equally to this work.

Authors and Affiliations

  1. College of Life Sciences, Key Lab of Plant Biotechnology in Universities of Shandong Province, Qingdao Agricultural University, Qingdao, 266109, China

    Qianqian Zhang, Jing Dong, Chunmei Cai & Shuai Li

  2. Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Sciences, Huzhou University, Huzhou, 313000, China

    Zhanqi Wang

  3. College of Agronomy, Qingdao Agricultural University, Qingdao, 266109, China

    Hong Zhu

  4. College of Plant Science, Jilin University, Changchun, 130062, China

    Ying Wang

Authors
  1. Qianqian Zhang
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  2. Jing Dong
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  3. Zhanqi Wang
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  4. Ying Wang
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  5. Chunmei Cai
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  6. Hong Zhu
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  7. Shuai Li
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Contributions

The project was conceived and designed by HZ and SL. QZ, JD, YW, and CC performed the experiments. QZ, JD, ZW, YW and CC analyzed the data. ZW, HZ and SL wrote the manuscript.

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Correspondence to Hong Zhu or Shuai Li.

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Communicated by Sergio J. Ochatt.

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Zhang, Q., Dong, J., Wang, Z. et al. Mungbean VrCOL1 regulates flowering time under short-day conditions in Arabidopsis. Plant Cell Tiss Organ Cult 148, 599–608 (2022). https://doi.org/10.1007/s11240-021-02217-w

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