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Modulation of quinonemethide triterpenes biosynthesis in Monteverdia floribunda (Reissek) biral root cultures by exogenous inhibitors

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Abstract

Quinonemethide triterpenes (QTs) are natural compounds that present anti-cancer action and are valuable chemotaxonomic markers in the Celastraceae plant family. However, the main obstacle to produce these compounds on a large scale is their low quantity in tissues of plants grown in situ. In addition, these molecules show a higher complex chemical structure and it is difficult to find strategies for enhancing their synthesis. In this context, the goal of this work was to evaluate the effect of inhibitors terbinafine, ancymidol, paclobutrazol, gibberellin (GA), and the association of GA + paclobutrazol on QT production from Monteverdia floribunda roots in vitro. After establishment of the total growth of the root system, the roots were subjected to the following inducing agents: ancymidol, uniconazole, paclobutrazol, GA, and terbinafine hydrochloride. Having established that in vitro roots from M. floribunda could produce QTs, the roots were inoculated in liquid WP medium with sodium acetate-1-13C and sodium acetate-2-13C. The results showed that ancymidol, GA, and the association of GA with paclobutrazol produced the best results obtained during QT elicitation. The growth regulator GA has a positive effect on the production of terpenoids from the mevalonate (MVA) pathway and it was confirmed that maytenin biosynthesis occurs via the MVA pathway.

Key message

The goal of this work was to evaluate the effect of inhibitors on quinonemethide triterpenes production from M. floribunda roots in vitro, besides the biosynthetic route was confirmed.

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Acknowledgements

The authors are grateful to the Biotechnology Unit (UNAERP). M.V. also thanks CAPES for the award of scholarships. The authors would like to thank Dr. Nivaldo Boralle (IQ/UNESP, Araraquara, SP, Brazil) for NMR analyses.

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Authors and Affiliations

  1. Biotechnology Unit, Universidade de Ribeirão Preto (UNAERP), Ribeirao Preto, SP, Brazil

    Mayara Valdevite, Bianca W. Bertoni, Suzelei de C. França, Ana Maria S. Pereira & Adriana A. Lopes

  2. Botanic Garden of Medicinal Plant Ordem e Progresso (BOP), Jardinópolis, SP, Brazil

    Bianca W. Bertoni & Ana Maria S. Pereira

  3. Faculty of Philosophy, Sciences and Letters at Ribeirão Preto, Universidade de São Paulo (USP), Ribeirao Preto, SP, Brazil

    Eduardo J. Crevelin

  4. Universidade tecnológica Federal do Paraná (UTFPR), Curitiba, PR, Brazil

    Leonardo Biral

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  1. Mayara Valdevite
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  4. Leonardo Biral
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Contributions

The author’s contribution was changed: AMSP designed research; MV, BWB, EJC, LB and AAL: performed research; AMSP, BWB, AAL, and SCF analyzed data; AMSP and AAL: wrote the manuscript and all authors reviewed the manuscript.

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Correspondence to Ana Maria S. Pereira or Adriana A. Lopes.

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Communicated by Christophe Hano.

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This article belongs to the Topical Collection: Secondary Metabolites and Medicinal Plant Biotechnology.

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Valdevite, M., Bertoni, B.W., Crevelin, E.J. et al. Modulation of quinonemethide triterpenes biosynthesis in Monteverdia floribunda (Reissek) biral root cultures by exogenous inhibitors. Plant Cell Tiss Organ Cult 149, 313–324 (2022). https://doi.org/10.1007/s11240-021-02214-z

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  • DOI: https://doi.org/10.1007/s11240-021-02214-z

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