Abstract
The current investigation revealed a highly proficient and reproducible method for comprehensive in vitro regeneration and structural differentiation of bulblets in Crinum malabaricum Lekhak & Yadav using meta-topolin [mT; 6-(3-hydroxybenzylamino) purine] [6-benzylaminopurine] for the first time. Cultures were established from twin scales explants using optimized sterilization method and growth regulators for regeneration of in vitro plantlets. It was observed that mT at 2.0 mg L−1 was the main factor involved in direct bulblets formation from the basal plate, and the highest mean number of bulblets per twin scale explant was 26 within 8 weeks. On maturation, the bulblets were rooted simultaneously on the mT containing medium. Regenerated plantlets were acclimatized with a 100% survival rate after 8 weeks. Light microscopic examination of bulblet scales/cataphylls was done to characterize the in vitro bulblets developed from mT and BAP containing media. The bulblet scales differed in quantitative traits of tissue systems, and the meta-topolin derived bulblets represented well differentiated dermal, ground and vascular tissues. The results obtained may provide a new tool to improve the production of pharmaceutically important compounds (viz. galanthamine) in bulblets and give the guarantee of survival of plants under natural conditions which can reaffirm the conservation measure of this critically endangered medicinal plant.
Key message
Meta-topolin enhanced in vitro production of bulblets with structurally stable cataphylls in Crinum malabaricum.
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Data sharing not applicable to this article as no datasets were generated during the current study.
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Acknowledgements
The authors are grateful to the Science and Engineering Research Board, Department of Science and Technology, Government of India (Sanction Number EMR/2016/007795) for providing financial support to undertake the work for in vitro conservation of this critically endangered medicinal plant.
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MM, MSS, and AD: Conceptualization, investigation, methodology. PS and CRM: Data compilation and hardening of the plants. MSS and MM: Writing of original draft and revision of the manuscript. All authors have read and approved the final manuscript.
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Manokari, M., Priyadharshini, S., Cokulraj, M. et al. Meta-topolin induced morphometric and structurally stable bulblets in Malabar River Lily (Amaryllidaceae). Plant Cell Tiss Organ Cult 148, 377–385 (2022). https://doi.org/10.1007/s11240-021-02195-z
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DOI: https://doi.org/10.1007/s11240-021-02195-z