Abstract
Peanut (Arachis hypogaea L.) hairy roots are a potential tool for stilbene compound production. This study focuses on the most efficient elicitation strategy for inducing stilbene compound production, especially for the prenylated forms of trans-arachidin-1 and trans-arachidin-3. To the best of our knowledge, this is the first report of simultaneous treatment involving chitosan (CHT), methyl jasmonate (MeJA), and cyclodextrin (CD); CHT + MeJA + CD in peanut hairy roots to induce substantial amounts of trans-arachidin-1 and trans-arachidin-3 at 72 h of the elicitation period. The results demonstrate the highest amounts of trans-arachidin-1 and trans-arachidin-3, with 684.30 ± 183.85 and 543.94 ± 171.17 mg/g dry weight of hairy roots, respectively. The highest antioxidant capacity determined by ABTS and FRAP assays was 188.95 ± 0.43 µmol Trolox/g dry weight of hairy roots and 24.61 ± 0.50 mg ascorbic acid/g dry weight of hairy roots, respectively, while the highest phenolic content was also detected in this crude extract, with 8.67 ± 0.11 mg gallic acid/g dry weight of hairy roots. The antioxidant and pro-oxidant activity of the CHT + MeJA + CD crude extract in the protection and damage of DNA is of great interest and can have major positive impacts on health promotion and disease prevention.
Key message
Chitosan, methyl jasmonate, and cyclodextrin induce high stilbene compounds. The antioxidant in crude extract could scavenge free radicals and protect DNA, while pro-oxidant activity causes severe damage to genomic DNA.
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Abbreviations
- AAE:
-
Ascorbic acid equivalent
- ABTS:
-
2,2′-Azino-di-(3-ethylbenzthiazoline sulfonic acid)
- Cd:
-
Cadmium
- CD:
-
Cyclodextrin
- CHT:
-
Chitosan
- EDTA:
-
Ethylenediamine tetraacetic acid
- EtOH:
-
Ethanol
- FeCl3 :
-
Ferric chloride
- FRAP:
-
Ferric reducing antioxidant power
- GAE:
-
Gallic acid equivalent
- H2O2 :
-
Hydrogen peroxide
- HPLC:
-
High-performance liquid chromatography
- MeJA:
-
Methyl jasmonate
- NaCl:
-
Sodium chloride
- NO:
-
Nitric oxide
- OH• :
-
Hydroxyl radical
- O2•− :
-
Superoxide anion radical
- PQ:
-
Paraquat
- PR:
-
Pathogenesis-related protein
- ROS:
-
Reactive oxygen species
- SDS:
-
Sodium dodecyl sulfate
- TEAC:
-
Trolox equivalent antioxidant capacity
- TPTZ:
-
2,4,6-Tri(2-pyridyl)-s-triazine
- UV:
-
Ultraviolet
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Acknowledgements
We acknowledge Assoc. Prof. Dr. Sermsiri Chanprame (Kasetsart University, THAILAND) for initial support of agrobacterium culture in hairy root experiment.
Funding
This work was supported by National Research Council of Thailand 2020 [grant number NRCT5-RSA63011-03]; and in part by the graduate research grant from the Agricultural Research Development Agency (Public Organization), Thailand 2020.
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PC: Conceptualization, methodology, formal analysis. WP: Methodology, validation, data curation CP: Methodology, formal analysis. OI: Methodology. AK: Project administration, supervision. AL: Project administration, supervision, visualization, writing original draft; writing—review & editing.
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Chayjarung, P., Poonsap, W., Pankaew, C. et al. Using a combination of chitosan, methyl jasmonate, and cyclodextrin as an effective elicitation strategy for prenylated stilbene compound production in Arachis hypogaea L. hairy root culture and their impact on genomic DNA. Plant Cell Tiss Organ Cult 147, 117–129 (2021). https://doi.org/10.1007/s11240-021-02112-4
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DOI: https://doi.org/10.1007/s11240-021-02112-4