Abstract
In vitro cultures from Mammillaria candida and Turbinicarpus laui synthesize a wide range of antioxidant compounds, which biological effects have not been studied yet. Therefore, the aim of this work was to analyze if the metabolites present in M. candida and T. laui in vitro cultures exhibit vasorelaxant activities in aorta rings. The evaluation was performed using extracts from irradiated calluses, chitosan-elicited cellular suspensions, and their respective controls. The highest vasodilation was observed in extracts from irradiated callus of M. candida (99%) and elicited suspensions of T. laui (92%). To elucidate the mechanisms involved on the vasodilation, additional experiments were performed in the presence of NG-nitro-l-arginine methyl ester, indomethacin, tetraethylammonium chloride and atropine. The vasodilation mediated by T. laui metabolites was associated to endothelium nitric oxide synthase, prostaglandin I2, K+ channels and muscarinic receptors activation, while that of M. candida was partially mediated by K+ channels. These results demonstrate that in vitro cultures of M. candida and T. laui are promising sources of pharmaceutical compounds for hypertension treatment.
Key message
Metabolites present on cacti in vitro cultures exerted more than 90% vasodilation on aorta ring. These cultures could be considered as a viable and efficient source of compounds for the treatment of hypertension.
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Acknowledgements
We are grateful to CONACYT for the scholarship (No. 401860) to ARM, to Dra. Daniela Salado-Leza for the English review and to Dr. Ricardo Espinosa-Tanguma for his technical support.
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AR realized the experimental work, participated on elaboration of tables and figures and manuscript. JR and CG participates in the design of vasorelaxation experiments and discussion of results. MSS is the leader of the group, designed the project and experimental work, participates in revision, discussion of results and wrote the paper.
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The animals used in this work were handled according to the Committee of Ethics in Research and Teaching of the UASLP (protocol CEID2014032).
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Communicated by So-Young Park.
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Reyes-Martínez, A., Valle-Aguilera, J.R., Gonzalez, C. et al. Vasorelaxant activity of metabolites present in Mammillaria candida and Turbinicarpus laui in vitro cultures. Plant Cell Tiss Organ Cult 147, 9–20 (2021). https://doi.org/10.1007/s11240-021-02100-8
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DOI: https://doi.org/10.1007/s11240-021-02100-8