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Induction and characterization of tetraploids in poplar

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Abstract

Tetraploid poplar plants were induced by colchicine from tissue-cultured shoots; the induction efficiency varied with colchicine concentration. Morphologically, induced tetraploid plants were significantly distinguishable from diploid plants; their leaves were larger, thicker, and rounder, with larger stomata and more chloroplast numbers but smaller stomata index in the abaxial leaf epidermis. Data from validation with chromosome counting and flow cytometry corresponded well with changes in stomata size and chloroplast number. This ready use protocol would facilitate poplar breeding.

Key message

Tetraploid induction by colchicine in Populus euroamericana from tissue cultured shoots.

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Authors and Affiliations

  1. Bestline Biotechnology Company Ltd, Shanghai, 200240, China

    Xiaofang Yan, Jie Zhang & Hui Zhang

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  1. Xiaofang Yan
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  2. Jie Zhang
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  3. Hui Zhang
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Correspondence to Xiaofang Yan.

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Klaus Eimert.

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Yan, X., Zhang, J. & Zhang, H. Induction and characterization of tetraploids in poplar. Plant Cell Tiss Organ Cult 146, 185–189 (2021). https://doi.org/10.1007/s11240-021-02043-0

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  • DOI: https://doi.org/10.1007/s11240-021-02043-0

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