Skip to main content
SpringerLink
Log in

Morpho-physio-biochemical traits of Hymenocrater longiflorus Benth.: a practical assessment of the interaction between salicylic acid and simvastatin under in vitro conditions

  • Original Article
  • Published:
Plant Cell, Tissue and Organ Culture (PCTOC) Aims and scope Submit manuscript

Abstract

Unlike the common plants, micropropagation of Hymenocrater longiflorus Benth. (Sura-halala) under in vitro conditions has neither been accurately set nor commercialized thus far. Similarly, there is no study available to address the impacts of phytohormones on this plant; therefore, this study was conducted to appraise the effects of salicylic acid (SA) at three levels (0, 0.5, and 1 mM) and simvastatin (SV) at four levels (0, 5, 10, and 20 μM) on the morpho-physiological and biochemical features of Sura-halala under in vitro propagation. Accordingly, SA application increased shoot and root length, shoot and root dry weight, total soluble protein, total soluble carbohydrate, and photosynthetic pigment contents. The results indicated that 0.5 mM application of SA resulted in the highest means of these traits, under almost all SV levels. Besides, SV application under a constant level of SA, either one of 0, 0.5 or 1 mM, induced the length and number of regenerated shoots, but reduced the root regeneration ability. Our final results indicated that application of low concentration of SV (5 μM) coupled with the medium concentration of SA (around 0.5 mM) was an appropriate treatment combination to obtain a higher production of Sura-halala under in vitro propagation. Also, the results indicated the requirement of further investigation on the interaction effect of SA and SV, at lower concentrations, together with other plant growth regulators (PGR) on Sura-halala production under in vitro propagation.

Key message

SA enhance the growth of Sura-halala, and improved the morphological traits. SV application inhibited root regeneration. SV improve growth parameters under a constant amount of SA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Ali HEM, Ismail GSM (2014) Tomato fruit quality as influenced by salinity and nitric oxide. Turk J Bot 38:122–129

    Article  CAS  Google Scholar 

  • Baninasab B, Baghbanha MR (2013) Influence of salicylic acid pre-treatment on emergence and early seedling growth of cucumber (Cucumis sativus) under salt stress. Int J Plant Prod 5:345

    Google Scholar 

  • Belin C, Thomine S, Schroeder JI (2009) Water balance and the regulation of stomatal movements, abiotic stress adaptation in plants. Springer, Berlin, pp 283–305

    Book  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  • Çanakçi S (2008) Effects of salicylic acid on fresh weight change, chlorophyll and protein amounts of radish (Raphanus sativus L.) seedlings. J Biol Sci 8:431–435

    Article  Google Scholar 

  • Divya P, Puthusseri B, Neelwarne B (2014) The effect of plant regulators on the concentration of carotenoids and phenolic compounds in foliage of coriander. LWT Food Sci Technol 56:101–110

    Article  CAS  Google Scholar 

  • Dong CJ, Wang XL, Shang QM (2011) Salicylic acid regulates sugar metabolism that confers tolerance to salinity stress in cucumber seedlings. Sci Hortic 129:629–636

    Article  CAS  Google Scholar 

  • Fereidoonfar H, Salehi Arjmand H, Khadivi A, Akramian M (2018) Morphological variability of sumac (Rhus coriaria L.) germplasm using multivariate analysis. Ind Crops Prod 120:162–170

    Article  Google Scholar 

  • Folin O, Ciocalteu V (1927) On tyrosine and tryptophane determinations in proteins. J Biol Chem 73:627–650

    Article  CAS  Google Scholar 

  • Ghaderi N, reza Hatami M, Mozafari A, Siosehmardeh A (2018) Change in antioxidant enzymes activity and some morpho-physiological characteristics of strawberry under long term salt stress. Physiol Mol Biol Plants 24:833–843

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gharib FA (2006) Effect of salicylic acid on the growth, metabolic activities and oil content of basil and marjoram. Int J Agric Biol 4:485–492

    Google Scholar 

  • Hossain MA, Bhattacharjee S, Armin SM et al (2015) Hydrogen peroxide priming modulates abiotic oxidative stress tolerance: insights from ROS detoxification and scavenging. Front Plant Sci 6:420

    Article  PubMed  PubMed Central  Google Scholar 

  • Hosseini S, Mashayekhi K (2011) The effects of salicylic acid on induction somatic embryogenesis and plant pigments carrot of root secondary phloem and petiole explant in b5 medium. J Agric Sci Nat 4:56–78

    Google Scholar 

  • Howlett BJ (2006) Secondary metabolite toxins and nutrition of plant pathogenic fungi. Curr Opin Plant Biol 9:371–375

    Article  CAS  PubMed  Google Scholar 

  • Irigoyen J, Einerich D, Sánchez-Díaz M (1992) Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiol Plant 84:55–60

    Article  CAS  Google Scholar 

  • Jabbarzadeh Z, Khosh Khui M, Salehi H (2009) The effect of foliar applied salicylic acid on flowering of African violet. Aust J Basic Appl Sci 3:4693–4696

    Google Scholar 

  • Javaheri M, Mashayekhi K, Dadkhah A, Tavallaee FZ (2012) Effects of salicylic acid on yield and quality characters of tomato fruit (Lycopersicum esculentum Mill.). Int J Agric Crop Sci 4:1184–1187

    Google Scholar 

  • Khan W, Prithiviraj B, Smith DL (2003) Photosynthetic responses of corn and soybean to foliar application of salicylates. J Plant Physiol 160:485–492

    Article  CAS  PubMed  Google Scholar 

  • Khodary SEA (2004) Effect of salicylic acid on the growth, photosynthesis and carbohydrate metabolism in salt stressed maize plants. Int J Agric Biol 6:5–8

    CAS  Google Scholar 

  • Laule O, Fürholz A, Chang HS et al (2003) Crosstalk between cytosolic and plastidial pathways of isoprenoid biosynthesis in Arabidopsis thaliana. Proc Natl Acad Sci 100:6866–6871

    Article  CAS  PubMed  Google Scholar 

  • Lei T, Feng H, Sun X et al (2010) The alternative pathway in cucumber seedlings under low temperature stress was enhanced by salicylic acid. Plant Growth Regul 60:35

    Article  CAS  Google Scholar 

  • Lichtenthaler HK, Buschmann C (2001) Chlorophylls and carotenoids: measurement and characterization by UV-VIS spectroscopy. Curr Protoc Food Anal Chem 1:F4.3.1–F4.3.8

    Article  Google Scholar 

  • Moharekar ST, Lokhande SD, Hara T et al (2003) Effect of salicylic acid on chlorophyll and carotenoid contents of wheat and moong seedlings. Photosynthetica 41:315

    Article  CAS  Google Scholar 

  • Mozafari A, Havas F, Ghaderi N (2018) Application of iron nanoparticles and salicylic acid in in vitro culture of strawberries (Fragaria × ananassa Duch.) to cope with drought stress. Plant Cell, Tissue Organ Cult 132:511–523

    Article  CAS  Google Scholar 

  • Mozafari A, Ghaderi N, Havas F, Dedejani S (2019) Comparative investigation of structural relationships among morpho-physiological and biochemical properties of strawberry (Fragaria × ananassa Duch.) under drought and salinity stresses: a study based on in vitro culture. Sci Hortic 256:108601

    Article  CAS  Google Scholar 

  • Paek KY, Murthy HN, Zhong JJ (2014) Production of biomass and bioactive compounds using bioreactor technology. Springer, New York

    Book  Google Scholar 

  • Popova L, Ananieva E, Hristova V et al (2003) Salicylic acid and methyl jasmonate induced protection on photosynthesis to paraquat oxidative stress. Bulg J Plant Physiol 133:152

    Google Scholar 

  • Qaderi A, Akbari Z, Kalateh Jari S et al (2016) Improving trigonelline production in hairy root culture of fenugreek (Trigonella foenumgraecum). J Med Plants 3:73–80

    Google Scholar 

  • Rodrı́guez-Concepción M, Gruissem W (1999) Arachidonic acid alters tomato HMG expression and fruit growth and induces 3-hydroxy-3-methylglutaryl coenzyme A reductase independent lycopene accumulation. Plant Physiol 119:41–48

    Article  PubMed  PubMed Central  Google Scholar 

  • Saed-Moucheshi A, Fasihfar E, Hasheminasab H, Rahmani A, Ahmadi A (2013) A review on applied multivariate statistical techniques in agriculture and plant science. Int J Agron Plant Prod 4:127–141

    Google Scholar 

  • Saed-Moucheshi A, Shekoofa A, Pessarakli M (2014) Reactive oxygen species (ROS) generation and detoxifying in plants. J Plant Nutr 37:1573–1585

    Article  CAS  Google Scholar 

  • Saed-Moucheshi A, Razi H, Dadkhodaie A, Ghodsi M, Dastfal M (2019) Association of biochemical traits with grain yield in triticale genotypes under normal irrigation and drought stress conditions. Aust J Crop Sci 13:272–281

    Article  CAS  Google Scholar 

  • Saha DC, Padhy PK (2011) Effects of stone crushing industry on Shorea robusta and Madhuca indica foliage in Lalpahari forest. Atmos Pollu Res 2:463–476

    Article  CAS  Google Scholar 

  • Shahba Z, Baghizadeh A, Yosefi M (2010) The salicylic acid effect on the tomato (Lycopersicum esculentum Mill.) germination, growth and photosynthetic pigment under salinity stress (NaCl). J Stress Physiol Biochem 6:4–16

    Google Scholar 

  • Shahriari S, Khanahmadi M, Tahvilian R (2013) The study of essential oil of Hymenocrater longiflorus Benth growing in Paveh. J Rep Pharm Sci 2:111–115

    Google Scholar 

  • Silva EC, Nogueira R, Silva MA, Albuquerque MB (2011) Drought stress and plant nutrition. Plant Stress 5:32–41

    Google Scholar 

  • Singh B, Usha K (2003) Salicylic acid induced physiological and biochemical changes in wheat seedlings under water stress. Plant Growth Regul 39:137–141

    Article  CAS  Google Scholar 

  • Tandoori A, Ghasemnejad A, Alizadeh M (2015) Effect of methyl jasmonate and salicylic acid on‎ morphological characters internal pigments of artichoke callus. J Crops Improv 13

  • Toor RK, Savage GP (2005) Antioxidant activity in different fractions of tomatoes. Food Res Int 38:487–494

    Article  CAS  Google Scholar 

  • Towler MJ, Weathers PJ (2007) Evidence of artemisinin production from IPP stemming from both the mevalonate and the nonmevalonate pathways. Plant Cell Rep 26:2129–2136

    Article  CAS  PubMed  Google Scholar 

  • Wang K, Jin P, Cao S et al (2009) Methyl jasmonate reduces decay and enhances antioxidant capacity in Chinese bayberries. J Agric Food Chem 57:5809–5815

    Article  CAS  PubMed  Google Scholar 

  • Yin C, Wang X, Duan B et al (2005) Early growth, dry matter allocation and water use efficiency of two sympatric Populus species as affected by water stress. Environ Exp Bot 53:315–322

    Article  Google Scholar 

  • Zeki AA, Franzi L, Last J, Kenyon NJ (2009) Simvastatin inhibits airway hyperreactivity: implications for the mevalonate pathway and beyond. Am J Respir Crit Care Med 180:731–740

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhaohui Y (2006) Effect of SA on the physiological change and floresoence of chrysanthemum. J Anhui Agric Sci 34:4225

    Google Scholar 

  • Zhou LG, Wu JY (2006) Development and application of medicinal plant tissue cultures for production of drugs and herbal medicinal in China. Nat Prod Rep 23:789–810

    Article  CAS  PubMed  Google Scholar 

Download references

Funding

This work was supported by the University of Kurdistan under Grant number GRC96-00228-2.

Author information

Authors and Affiliations

  1. Department of Horticultural Sciences, Faculty of Agriculture, University of Kurdistan, P. O. Box: 416, 66177-15175, Sanandaj, Iran

    Maryam Hoseiny, Ali akbar Mozafari & Farzad Nazari

Authors
  1. Maryam Hoseiny
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ali akbar Mozafari
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Farzad Nazari
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

In this experiment A.M. designed experiment. M.H. performed experiment and assembled data under advice of A.M. and F.N.. A.M. supervised morphological and in vitro culture physiological experiments and measurement. Data analysis has been performed by A.M. with the help of F.N. and M.H. results discuss and write of manuscript was performed by A.M. and F.N.

Corresponding author

Correspondence to Ali akbar Mozafari.

Additional information

Communicated by Ranjith Pathirana.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Hoseiny, M., Mozafari, A.a. & Nazari, F. Morpho-physio-biochemical traits of Hymenocrater longiflorus Benth.: a practical assessment of the interaction between salicylic acid and simvastatin under in vitro conditions. Plant Cell Tiss Organ Cult 144, 607–617 (2021). https://doi.org/10.1007/s11240-020-01982-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-020-01982-4

Keywords

Search

Navigation