Abstract
In vitro micropropagation protocol for Basella rubra regeneration was tried through proliferation of axillary shoots of the potted mature plant. The improved seed germination (70%) was recorded upon 2% urea treatment. The nodal shoot segments from matured potted plant were used to initiate the multiple shoot proliferation. The shoot segments exhibited 70% shoot initiation when cultured on Murashige and Skoog (MS) medium supplemented with Indole-3-acetic acid (IAA) + N6 – Benzylaminopurine (BAP) (0.25 + 2.0 mg/L) and BAP + Kinetin (Kin) (2.0 + 0.5 mg/L) respectively. Multiple shoots (5–6) were obtained on MS medium supplemented with BAP + Kin and IAA + BAP respectively. When compared with silver nitrate (AgNO3) (2–40 µM) and activated charcoal (AC) (0.1–1.0%), the MS medium devoid of any plant growth regulator showed good number of shoots (5.48 ± 2.42), elongation (15.64 ± 2.42 cm) and root length (14.52 ± 2.78 cm). Upon transferring of regenerated microshoots to MS medium, simultaneous elongation of shoots with more shoot number, shoot length and rooting was achieved during four subcultures that carried out at 6 weeks’ interval. The regenerated in vitro shoots showed 100% rooting in MS medium and also in MS medium supplemented with 0.1–1.0% AC. Hundred percent survival of micropropagated shoots well rooted was established successfully under greenhouse condition and the plants were subsequently acclimatized and transferred to the field conditions wherein 90% success rate was noted.
Key message
We established a novel, and efficient protocol for in vitro B. rubra shoot cultures further multiplication in MS basal medium without any plant growth regulators with 100% elongation and rooting of microshoots that will overcome the use of synthetic hormones for improved micropropagation.
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Acknowledgements
The authors are thankful to CSIR New Delhi for the award of SRF fellowship and Department of Biotechnology (BT/PR1238/FNS/20/524/2011), Government of India, New Delhi for financial assistance. We greatly acknowledge the Director, CSIR-CFTRI for his kind support.
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Communicated by Sergey V Dolgov.
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Kumar, S.S., Giridhar, P. In vitro micropropagation of Basella rubra L. through proliferation of axillary shoots. Plant Cell Tiss Organ Cult 144, 477–483 (2021). https://doi.org/10.1007/s11240-020-01960-w
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DOI: https://doi.org/10.1007/s11240-020-01960-w