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Overexpression of ICE1 gene in mungbean (Vigna radiata L.) for cold tolerance

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Abstract

The putative transgenic mungbean (Vigna radiata Var. OBGG-52) plants were regenerated on Murashige and Skoog (1962) medium supplemented with 1.0 mg/L 6-benzylaminopurine (BA). The transgenic status of transformants (T2 generation) was determined through PCR analysis using nptII and ICE1 gene-specific primers as well as uidA reporter gene expression. About 75% of the transformed plantlets were successfully established in the greenhouse. Overexpression of ICE1 gene in transgenic mungbean plants resulted in cold-tolerance at the seedling stage when compared to non-transformed plants. Seeds from T2 generation were further studied for cold tolerance through nutrient culture. Transgenic seeds had significantly increased germination and growth on a nutrient medium at 10–14 °C in growth chamber compared to the non-transgenic control. Moreover, the root and shoot length of transformed plants were significantly increased at 10–14 °C as compared with the non-transformed plants. Under cold stress, it was also observed that the transformed plantlets have significantly higher chlorophyll, proline, and lipid content as well as anti oxidative enzyme activity as compared to non-transformed plants. This study will help in mungbean improvement program for abiotic stress tolerance.

Key message

Cold temperature damaged the crop productivity of mungbean (Vigna radiata L. Wilczek), a high protein rich legume crop. The overexpression of cold responsive transcription factor, INDUCER OF CBF EXPRESSION 1 (ICE1) in mungbean enhanced cold tolerance.

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Acknowledgements

The authors wish to acknowledge to Department of Biotechnology, Govt. of India for providing infrastructural facility and fund for research and FIST grant, DST, Govt. of India. The authors wish to acknowledge to Dr. L. Sahoo, Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati providing ICE1gene construct for the experiment.

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Authors and Affiliations

  1. Department of Agricultural Biotechnology, College of Agriculture, Odisha University of Agriculture & Technology, Bhubaneswar, Odisha, 751003, India

    Gyana Ranjan Rout, Dhaneswar Swain & Kundansigh Rajpalsingh Jadhao

  2. Biointeractions and Crop Protection, Rothamsted Research, West Common, Harpenden, Hertfordshire, AL5 2JQ, UK

    Anuradha Bansal

  3. Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati, 781039, India

    Rahul G. Shelke

  4. Department of Biochemistry, Central University of Rajasthan, Ajmer, 305817, India

    Sanjib Kumar Panda

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  1. Gyana Ranjan Rout
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Contributions

GRR & SKP design the experiment, DS, RGS & KRJ conducted the experiments and data preparation, GRR prepared the final draft and AB and SKP have fully edited the manuscript.

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Correspondence to Gyana Ranjan Rout.

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Communicated by Sergio J. Ochatt.

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Rout, G.R., Bansal, A., Swain, D. et al. Overexpression of ICE1 gene in mungbean (Vigna radiata L.) for cold tolerance. Plant Cell Tiss Organ Cult 143, 593–608 (2020). https://doi.org/10.1007/s11240-020-01944-w

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