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Growth and physiological characteristics of wasabi plantlets cultured by photoautotrophic micropropagation at different temperatures

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Abstract

In this study, wasabi plantlets were grown by photoautotrophic micropropagation under nine different day/night temperature regimes ranging from 10 °C/10 to 26 °C/26 °C. On day 28 of the culture, the dry weight and the leaf area were found to be highest under a constant air temperature of 18 °C/18 °C. Partitioning of plant biomass into stems increased whilst partitioning into leaves decreased with the increase in temperature. Whereas, partitioning of plant biomass into roots increased when the temperature increased from 10 to 18 °C but decreased with further increase in temperature to 26 °C. The shoot/root ratio showed an opposite trend compared with partitioning into roots, whereas the chlorophyll content of leaves increased when the temperature increased from 10 to 26 °C. The net photosynthetic rate of whole plantlets showed the same trend as plantlet biomass and was the highest in 18 °C/14 °C, 18 °C/18 °C, and 22 °C/18 °C treatments. By contrast, the value of the net photosynthetic rate per unit leaf area decreased sharply at temperatures ranging from 10 to 18 °C. A linear relationship between the net photosynthetic rate and RGR was clearly evident because of the large differences in the leaf area values. The specific leaf area of wasabi plantlets decreased slightly at temperatures ranging from 10 to 18 °C but increased substantially within the range of 18–26 °C; however, the leaf dry matter content showed an opposite trend. Low- and high-temperature ranges had contrasting effects on the leaf mass area and leaf nitrogen concentration. There was the high descent in dissolved oxygen concentration of culture medium at all treatments from day 0 to 7.

Key message

Greater growth of wasabi plantlets in 18 °C/14 °C, 18 °C/18 °C, and 22 °C/18 °C treatments. Biomass allocation to leaves is more important than the net photosynthetic rate per leaf area for supporting a high relative growth rate.

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Abbreviations

DO:

Dissolved oxygen concentration

DW:

Dry weight

FW:

Fresh weight

LA:

Leaf area

LMA:

Leaf mass area

LMF:

Leaf mass fraction

LDMC:

Leaf dry matter content

Pn :

Net photosynthetic rate

PA:

Photoautotrophic micropropagation

PM:

Photomixotrophic micropropagation

RMF:

Root mass fraction

RGR:

Relative growth rate

SMF:

Stem mass fraction

SLA:

Specific leaf area

S/R:

Shoot/root dry weight ratio

SRL:

Specific root length

WC:

Water content

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Acknowledgements

This research was partly supported by the Japan Society for Promotion of Science (JSPS Grants-in-Aid for Scientific Research, 15K12246 and 18H04984). The authors would like to thank Yanmar Co. Ltd. for providing wasabi materials and equipment and also Dr. Hajime Furukawa, Osaka Prefecture University, for providing meristem culture technique.

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Authors and Affiliations

  1. Department of Science, Technology and International Project, Ho Chi Minh City University of Technology (HUTECH), Ho Chi Minh City, Vietnam

    Nhung Ngoc Hoang

  2. Department of Environmental Sciences and Technology, Osaka Prefecture University, Sakai, Osaka, 599-8531, Japan

    Yoshiaki Kitaya, Toshio Shibuya & Ryosuke Endo

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  1. Nhung Ngoc Hoang
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  2. Yoshiaki Kitaya
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  3. Toshio Shibuya
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  4. Ryosuke Endo
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Contributions

HNN conceived and designed research, conducted experiments, collected and analyzed the data, wrote the manuscript. KY conceived and designed research, revised the manuscript, approved the final version. ST and ER made technical supports and revised the manuscript.

Corresponding authors

Correspondence to Nhung Ngoc Hoang or Yoshiaki Kitaya.

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Conflict of interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Additional information

Communicated By Francisco de Assis Alves Mourão Filho.

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Hoang, N.N., Kitaya, Y., Shibuya, T. et al. Growth and physiological characteristics of wasabi plantlets cultured by photoautotrophic micropropagation at different temperatures. Plant Cell Tiss Organ Cult 143, 87–96 (2020). https://doi.org/10.1007/s11240-020-01898-z

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