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Overexpression of the mango MiCO gene delayed flowering time in transgenic Arabidopsis

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Abstract

CONSTANS (CO)/CONSTANS-like (COL) genes play an important role in the photoperiodic flowering pathway. However, the functional roles of the CO/COL genes in mango (Mangifera indica L.) remain unknown. In this study, the MiCO gene was isolated from mango and functionally characterized. The open reading frame is 966 bp in length and encodes 322 amino acids; the protein contains two conserved B-box zinc finger domains and a CCT domain and thus belongs to the CO/COL group I protein family. The MiCO protein fused to green fluorescent protein localized to the nucleus. MiCO was expressed in all tested organs and was highly expressed especially in the leaves and stems of nonflowering branches, but its expression decreased in flowering branches. The expression level of MiCO significantly increased during the transition from vegetative to reproductive growth. MiCO was also expressed in accordance with the circadian rhythm, with the greatest expression level detected at 09:00. Overexpression of MiCO in Arabidopsis significantly delayed flowering under both long-day and short-day conditions.

Key message

In this study, a MiCO gene from mango was characterized. Overexpression of MiCO delayed flowering in transgenic Arabidopsis. The MiCO gene may play an important role in the flowering process of mango.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (31860541 and 31660561), Science and Technology Major Project of Guangxi (GXKJ-AA17204026 and GXKJ-AA17204097-3), the Innovation Team of Guangxi Mango Industry Project (nycytxgxcxtd-06-02) and the State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources (SKLCUSA-a201906 and SKLCUSA-c201901).

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Author notes

  1. Yuan Liu, Cong Luo, and Xiu-Juan Zhang contributed equally to this work .

Authors and Affiliations

  1. College of Agriculture, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Guangxi, Nanning, 530004, China

    Yuan Liu, Cong Luo, Xiu-Juan Zhang, Xin-Xi Lu, Hai-Xia Yu, Xiao-Jie Xie, Zhi-Yi Fan, Xiao Mo & Xin-Hua He

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  1. Yuan Liu
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Contributions

CL and XHH designed and funded the experiments; YL, XJZ and HY performed the experiments; XXL, XJX, ZYF, and XM helped with the experiments and data analysis. YL and CL wrote the manuscript. All authors have read and approved the final version of the manuscript.

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Correspondence to Xin-Hua He.

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Communicated by Degao Liu.

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Liu, Y., Luo, C., Zhang, XJ. et al. Overexpression of the mango MiCO gene delayed flowering time in transgenic Arabidopsis. Plant Cell Tiss Organ Cult 143, 219–228 (2020). https://doi.org/10.1007/s11240-020-01894-3

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