Pennisetum × advena (purple fountain grass) is an ornamental grass with considerable commercial value. In vitro induction of hexaploids and evaluation of the morphological and anatomical traits in P. × advena were conducted in this study. Nine independent hexaploids were generated from calli, which was initiated from immature inflorescence tissue of triploid P. × advena, treated with 0.1% colchicine for 24 h. Their ploidy levels were first screened by flow cytometry and further identified by chromosome counting. Compared with the triploids, the hexaploids showed a significant delay in flowering, and reduction in plant height, the number of tillers and number of inflorescences per plant, leaf length and width, inflorescence diameter, and the number of florets per inflorescence, but an increase in floret fresh weight, and anther diameter and length. Pollen grains in the hexaploids were well developed, and their fertility was restored. The production of hexaploids of P. × advena by colchicine treatment, and assessment of their growth parameters and pollen fertility, provide important information for future breeding of P. × advena.
Nine independent hexaploids were generated from calli derived from immature inflorescence tissue of triploid Pennisetum × advena by a colchicine treatment, and their morphological and anatomical traits were evaluated.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Adaniya S, Shirai D (2001) In vitro induction of tetraploid ginger (Zingiber offcinale Roscoe) and its pollen fertility and germinability. Sci Hortic 88:277–287. https://doi.org/10.1016/S0304-4238(00)00212-0
Barbosa S, Vide LCD, Pereira AV, Abreu JCD (2007) Chromose duplication of triploid hybrids between elephantgrass and pearl millet. Bragantia 66(3):365–372
Campos JMS, Davide LC, Salgado CC et al (2009) In vitro induction of hexaploid plants from triploid hybrids of Pennisetum purpureum and Pennisetum glaucum. Plant Breed 128:101–104
Chae WB, Hong SJ, Gifford JM, Lane Rayburn A, Widholm JM, Juvik JA (2013) Synthetic polyploid production of Miscanthus sacchariflorus, Miscanthus sinensis, and Miscanthus × giganteus. GCB Bioenergy 5:338–350. https://doi.org/10.1111/j.1757-1707.2012.01206.x
Contreras RN, Owen J, Hanna WW, Schwartz B (2013) Evaluation of seven complex Pennisetum hybrids for container and landscape performance in the Pacific northwestern United States. HortTechnology 23:525–528
Dujardin M, Hanna WW (1989) Crossability of pearl millet with wild Pennisetum species. Crop Sci 29:77–80
Eng WH, Ho WS (2019) Polyploidization using colchicine in horticultural plants: a review. Sci Hortic 246:604–617. https://doi.org/10.1016/j.scienta.2018.11.010
Faleiro FG, Kannan B, Altpeter F (2016) Regeneration of fertile, hexaploid, interspecific hybrids of elephantgrass and pearl millet following treatment of embryogenic calli with antimitotic agents. Plant Cell Tissue Org Cult 124(1):57–67. https://doi.org/10.1007/s11240-015-0874-4
Guo J, Xu XM, Li WT, Zhu WY, Zhu HT, Liu ZQ, Luan X, Dai ZJ, Liu GF, Zhang ZM, Zeng RZ, Tang G, Fu XL, Wang SK, Zhang GQ (2016) Overcoming inter-subspecific hybrid sterility in rice by developing indica-compatible japonica lines. Sci Rep 6:26878. https://doi.org/10.1038/srep26878
Huang Z, Xu C, Li Y, Wang P, Li Y, Kang X (2015) Induction of somatic embryogenesis by anther-derived callus culture and plantlet ploidy determination in poplar (Populus × beijingensis). Plant Cell Tissue Org Cult 120:949–959. https://doi.org/10.1007/s11240-014-0649-3
Kermani MJ, Sarasan V, Roberts AV et al (2003) Oryzalin-induced chromosome doubling in Rosa and its effect on plant morphology and pollen viability. Theor Appl Genet 107:1195–1200. https://doi.org/10.1007/s00122-003-1374-1
Mu T, Zhang XY, Wang JG, Fan JP, Gong SF (2013) The optimization of Pennisetum Agrobacterium-mediated transformation system and obtaining of transgenic plants. Crops 1:45–48
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays for tobacco tissue cultures. Physiol Plant Int J Plant Biol 15:473–497
Owen WG, Lopez RG (2018) Propagation daily light integral and root-zone temperature influence rooting of single-internode Pennisetum × advena culm cuttings. HortScience 53(2):176–182. https://doi.org/10.21273/HORTSCI12455-17
Padhye SR, Cregg BM, Cameron AC (2008) Chilling sensitivity of stored purple fountain grass propagules. Postharvest Biol Technol 49(2):235–240. https://doi.org/10.1016/j.postharvbio.2008.01.022
Sattler MC, Carvalho CR, Clarindo WR (2016) The polyploidy and its key role in plant breeding. Planta 243:281–296. https://doi.org/10.1007/s00425-015-2450-x
Simpson CE, Bashaw EC (1969) Cytology and reproductive characteristics in Pennisetum setaceum. Am J Bot 56:31–36
Wei JL, Li LF, Yu XB (2015) Tissue culture and rapid propagation of Pennisetum setaceum ‘Rubrum’. Plant Physiol J 51(2):207–211
Widoretno W (2016) In vitro induction and characterization of tetraploid Patchouli (Pogostemon cablin Benth.) plant. Plant Cell Tissue Org Cult 125(2):261–267. https://doi.org/10.1007/s11240-016-0946-0
Wipff JK, Veldkamp JF (1999) Pennisetum advena sp. nov. (Poaceae: Paniceae): a common ornamental grass throughout the southern United States. Sida 18(4):1031–1036
Wood TE, Takebayashi N, Barker MS, Mayrose I, Greenspoon PB, Rieseberg LH (2009) The frequency of polyploid speciation in vascular plants. Proc Nat Acad Sci 106(33):13875–13879. https://doi.org/10.1073/pnas.0811575106
Xin JN, Xu W, Wang ZY, Sun C, Zhang ML (2013) Application of Pennisetum alopecuroides as ornamental grass. Grassland Turf 33(5):90–93
Yang ZY, Shen ZX, Tetreault H, Johnson L, Friebe B, Frazier T, Huang LK, Burklew C, Zhang XQ, Zhao BY (2014) Production of autopolyploid lowland switchgrass lines through in vitro chromosome doubling. Bioenergy Res 7(1):232–242. https://doi.org/10.1007/s12155-013-9364-x
Yu CY, Kim HS, Rayburn AL, Widholm JM, Juvik JA (2009) Chromosome doubling of the bioenergy crop, Miscanthus × giganteus. GCB Bioenergy 1(6):404–412. https://doi.org/10.1111/j.1757-1707.2010.01032.x
Yue YS, Zhu Y, Fan XF, Hou XC, Zhao CQ, Zhang S, Wu JY (2017) Generation of octoploid switchgrass in three cultivars by colchicine treatment. Ind Crops Prod 107:20–21. https://doi.org/10.1016/j.indcrop.2017.05.021
Zhang XY, Zhang HL, Mu T, Gong SF (2012) Effects of external hormones on induction and differentiation of callus of Chinese Pennisetum. Pratacult Sci 7:1066–1071
Zhang Y, Yuan XH, Teng WJ, Chen C, Liu H, Wu JY (2015) Karyotype diversity analysis and nuclear genome size estimation for Pennisetum Rich. (Poaceae) ornamental grasses reveal genetic relationship and chromosomal evolution. Sci Hortic 193:22–31. https://doi.org/10.1016/j.scienta.2015.06.018
Zhong XX, Liu ZW, Liu WG, Cui LL, Wu JZ, Zhang JL (2014) Analysis of characteristics of a hexaploid somatic mutant of hybrid Pennisetum. Acta Pratac Sin 23(4):107–113
This research was funded by the Scientific and Technological Innovation Capacity Construction Special Funds of the Beijing Academy of Agriculture and Forestry (KJCX20180101 and KJCX20170110), and the Project of Science and Technology, Beijing (171100007217001). We thank Robert McKenzie, Ph.D., from Liwen Bianji, Edanz Group China (www.liwenbianji.cn/ac), for editing the English text of a draft of this manuscript.
Conflict of interest
The authors declare that they have no competing interests.
Communicated by Alison M.R. Ferrie.
About this article
Cite this article
Yue, Y., Fan, X., Hu, Y. et al. In vitro induction and characterization of hexaploid Pennisetum × advena, an ornamental grass. Plant Cell Tiss Organ Cult 142, 221–228 (2020). https://doi.org/10.1007/s11240-020-01814-5
- Pennisetum × advena
- Flow cytometry