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Nitric oxide donor regulates Agrobacterium-mediated genetic transformation efficiency in soybean [Glycine max (L.) Merrill]

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Abstract

The present study investigates the potentiality of Sodium nitroprusside (SNP) to enhance the efficiency of genetic transformation in soybean. Half-seeds cultured on co-cultivation [4.44 μM N6-benzyl adenine (BA) and 30 μM SNP]; shoot induction (4.44 μM BA and 30 μM SNP) and rooting medium [4.93 μM indole 3-butyric acid (IBA) and 30 μM SNP] exhibited improved transformation efficiency (34.6%) in contrast to the regeneration system devoid of SNP (23%). The putatively transformed plants were evaluated by GUS assay and molecular analysis like PCR and Southern hybridization. Furthermore, the transformation system developed herein entails a shorter period (75-days) for developing plantlets from half-seeds of soybean. The outcome of this study revealed that the addition of SNP increased regeneration efficiency of plants, which translated to improved transformation efficiency in soybean.

Key message

This study is the first report employing sodium nitroprusside to enhance transformation efficiency in soybean.

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Acknowledgements

The authors acknowledge the Plant Molecular Biology Laboratory (PMBL), Department of Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India, for providing facilities to perform the experiments. First author, Mr. Sivabalan Karthik, extends his gratitude to Jawaharlal Nehru Memorial Fund (JNMF), New Delhi, India, for the grant of Jawaharlal Nehru Scholarship (Ref No: SU-1/88/2016-17/79) to perform his Doctoral work.

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Authors and Affiliations

  1. Department of Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620 024, India

    Sivabalan Karthik, Gadamchetty Pavan & Markandan Manickavasagam

Authors
  1. Sivabalan Karthik
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  2. Gadamchetty Pavan
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  3. Markandan Manickavasagam
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Contributions

SK planned the research and drafted the manuscript. MM supervised the study and reviewed the manuscript with GP. SK performed all the experiments and statistical analysis. All the authors have examined and recommended the final manuscript.

Corresponding author

Correspondence to Markandan Manickavasagam.

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Communicated by Sergio J. Ochatt.

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11240_2020_1808_MOESM1_ESM.docx

Supplementary Fig. 1 Linear map of the T-DNA region of binary vector pCAMBIA 1301-bar. Supplementary file1 (DOCX 250 kb)

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Karthik, S., Pavan, G. & Manickavasagam, M. Nitric oxide donor regulates Agrobacterium-mediated genetic transformation efficiency in soybean [Glycine max (L.) Merrill]. Plant Cell Tiss Organ Cult 141, 655–660 (2020). https://doi.org/10.1007/s11240-020-01808-3

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