Abstract
Here, we established a novel, efficient, and cost-effective rooting culture system for highbush blueberry (Vaccinium corymbosum). This method overcomes the inefficient, expensive, and time-consuming nature of traditional methods and provides a theoretical basis for the industrial rooting of micropropagated blueberry seedlings. Semisolid half-strength woody plant medium (1/2WPM), supplemented with carrageenan as fixing material, was packed into high molecular polypropylene plastic bags. Optimum response of ‘Ozarkblue’ was observed in 1/2WPM supplemented with 0.1 mg/l indole-3-butyric acid (IBA); 97.7% rooting rate was achieved at 45 days after culture, with the average root number and length reaching 6.5 ± 0.39 and 3.5 ± 0.16 cm, respectively. The highest rooting rate of ‘Weiwa Kuahuat’ (84.0%) was achieved with 0.5 mg/l IBA, resulting in a favorable average root number (3.0 ± 0.43) and length (3.0 ± 0.43 cm). ‘Sierra’ showed the highest rooting rate (100.0%) in the presence of 1.0 mg/l IBA, with an average root number and length of 5.9 ± 0.9 and 3.9 ± 0.62 cm, respectively. The earliest and fastest rooting was recorded when shoots were transplanted into bags for 13 days. A space utilization experiment showed that the density of well-grown blueberry seedlings achieved in bags (1600 seedlings/m2) was superior to that obtained using traditional in vitro and ex vitro rooting methods (270 and 420 seedlings/m2, respectively). This is the first report of a complete rooting system that could serve as a feasible blueberry rooting method with superior rooting time, rooting rate, medium consumption, space requirement, and portability.
Key message
We established a novel, efficient, and cost-effective rooting culture system for the micro-propagation of highbush blueberry seedlings that overcomes many of the drawbacks of traditional methods of industrial rooting.
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Acknowledgements
This study was funded by the Natural Science Foundation of Beijing, China (Grant No. 6172026 to L-YZ), and the National Natural Science Foundation of China (Grant No. 31872942 to L-YZ).
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YXG conducted the experiments and wrote the manuscript. YYZ and MZ analyzed the data. L-YZ conceived the study and approved the final manuscript.
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Communicated by Yu-Jin Hao.
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Guo, YX., Zhao, YY., Zhang, M. et al. Development of a novel in vitro rooting culture system for the micropropagation of highbush blueberry (Vaccinium corymbosum) seedlings. Plant Cell Tiss Organ Cult 139, 615–620 (2019). https://doi.org/10.1007/s11240-019-01702-7
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DOI: https://doi.org/10.1007/s11240-019-01702-7