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Absorption of zinc ions dissolved from zinc oxide nanoparticles in the tobacco callus improves plant productivity

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Abstract

Zinc oxide (ZnO) nanoparticles (NPs) are soluble in water and can release Zn2+, an essential mineral that promotes the growth of plant cells. When ZnO NPs were administered to tobacco (Nicotiana tabacum cv. Samusun-NN) callus under white light irradiation, a concentration-dependent increase in weight was observed. Specifically, an increase in chlorophyll levels was triggered by blue light and induced by ZnO NPs. mRNA-seq analysis showed that during the early stages of tobacco callus exposure to ZnO NPs and white light irradiation, there was considerable fluctuation in the expression of genes related to salt stress. After 24 h, the expression of cellular component and growth-related genes also fluctuated. Analysis by RT-qPCR revealed that, after 1 day of ZnO NPs exposure, the expression levels of photosynthesis-related genes were enhanced. The Zn content of control-treated callus was 0.19 mg g−1 dry weight, whereas that of callus cultured with the ZnO bulk particles (BPs), with a particle diameter of 2000 nm, was 2.59 mg g−1 dry weight, and for callus cultured with ZnO NPs, with a particle diameter 34 nm, the Zn content was 3.37 mg g−1 dry weight. These results indicate that ZnO particles supplied large amounts of Zn to the callus, suggesting that the smaller the particle size, the larger the surface area of particles dissolve zinc ions more efficiently and the more ions are supplied to tobacco callus cells, and resulting in an increase in plant productivity.

Key message

Under the light illumination, incubation of tobacco callus with zinc oxide nanoparticle dispersion resulted in supply of much zinc ions into cell, and induction of chlorophyll accumulation and cell proliferation.

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Acknowledgements

We thank Ms. Junko Muramatsu for her advice on the experimental design. This research was supported by Grants-in-Aid for Scientific Research (No. 18K19882) from the Japanese Society for the Promotion of Science.

Funding

This study was funded by Grants-in-Aid for Scientific Research from the Japanese Society for the Promotion of Science (no. 18K19882).

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Authors and Affiliations

  1. Department of Biological Science, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8531, Japan

    Shizue Yoshihara, Satomi Takeda & Hayato Tokumoto

  2. Department of Chemical Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8531, Japan

    Kasumi Yamamoto & Yoshino Nakajima

  3. Osaka Prefecture University College of Technology, 26-12 Saiwai-cho, Neyagawa, Osaka, 572-8572, Japan

    Kensuke Kurahashi

Authors
  1. Shizue Yoshihara
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  2. Kasumi Yamamoto
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  3. Yoshino Nakajima
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  4. Satomi Takeda
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  5. Kensuke Kurahashi
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  6. Hayato Tokumoto
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Contributions

SY: Study conception and design, Acquisition of data, Analysis and interpretation of data, Drafting manuscript. KY: Acquisition of data, Analysis and interpretation of data. YN: Acquisition of data, Analysis and interpretation of data. ST: Analysis and interpretation of data. KK: Analysis and interpretation of data. HT: Study conception and design, Analysis and interpretation of data, Drafting manuscript.

Corresponding author

Correspondence to Hayato Tokumoto.

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Communicated by Silvia Moreno.

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Yoshihara, S., Yamamoto, K., Nakajima, Y. et al. Absorption of zinc ions dissolved from zinc oxide nanoparticles in the tobacco callus improves plant productivity. Plant Cell Tiss Organ Cult 138, 377–385 (2019). https://doi.org/10.1007/s11240-019-01636-0

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  • DOI: https://doi.org/10.1007/s11240-019-01636-0

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