Abstract
The effect of several parameters on trans-resveratrol extracellular production in Vitis vinifera cv Monastrell suspension cultured cells elicited with cyclodextrins separately or in combination with methyl jasmonate was evaluated. The results indicated that trans-resveratrol production was independent of aeration surface when the same volume of suspension cultured cells was cultivated in flasks of different size (250, 500 and 1000 mL). Moreover, the effect of scale-up on trans-resveratrol production under elicitation was also evaluated. The highest trans-resveratrol production (2140 mg/L) was observed when 200 mL of suspension cultured cells were cultivated in flasks of 500 mL capacity in the presence of cyclodextrins and methyl jasmonate. Finally, the results also indicated that the same suspension cultured cells can be used for at least six continuous elicitation cycles with cyclodextrins and methyl jasmonate to enhance the production of trans-resveratrol.
Key message
trans-resveratrol production was independent of aeration surface and the same grapevine cells could be used for at least six continuous elicitation cycles to enhance the production of trans-resveratrol.
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Abbreviations
- CD:
-
Cyclodextrins
- FW:
-
Fresh weight
- MJ:
-
Methyl jasmonate
- SCC:
-
Suspension cultured cells
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Acknowledgements
This work was supported by the Ministerio de Economía y Competitividad (No. BIO2017-82374-R) and Fundación Seneca-Agencia de Ciencia y Tecnología de la Región de Murcia (No. 19876/GERM/15).
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Communicated by Klaus Eimert.
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Belchí-Navarro, S., Pedreño, M.A. & Almagro, L. Critical parameters on which the production of trans-resveratrol in Vitis vinifera cv Monastrell cell cultures depends. Plant Cell Tiss Organ Cult 138, 395–398 (2019). https://doi.org/10.1007/s11240-019-01632-4
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DOI: https://doi.org/10.1007/s11240-019-01632-4