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Lateral Organ Boundaries Domain 19 (LBD19) negative regulate callus formation in Arabidopsis

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Abstract

Callus is a remarkable regeneration tissue. The genes correlated with root development can be involved in regulating callus development in higher plants. Several Lateral Organ Boundaries Domain (LBD) transcription factors are known as the key regulators in callus induction, and they also participate in the development of lateral root. Here we took a systemic analysis approach to investigate the expression pattern of many LBD family members during callus induction on CIM medium. We found that most of the LBD members were upregulated on CIM medium but LBD19 was different from other family members showing increased expression at early stage but decreased expression later on. We were unable to obtain the loss of function mutant of lbd19 plant, so we generated the overexpression transgenic lines of LBD19. The overexpression of LBD19 caused obvious phenotypic changes in vegetative development and suppressed callus formation. The quantitative RT-PCR analysis results revealed that the expression of most genes that promote callus formation were down-regulated in callus of oeLBD19. These findings suggest that LBD19 may plays an important function in coordinating callus formation in Arabidopsis along with other LBD members.

Key message

1. Analysis the expression of LBD family genes during callus formation. 2. Over-expression of LBD19 affect callus formation and plant growth. 3. LBD19 plays an negative regulator in callus formation.

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Acknowledgements

This work was supported by the grants from the National Natural Science Foundation of China (31370214) and the Science and Technology Commission of Shanghai Municipality (18DZ2260500). The research was also partially sponsored by Shanghai Sailing Program (17YF1414000).

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Author notes

  1. Siqi Liu and Bo Wang contributed equally to this work.

Authors and Affiliations

  1. Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China

    Siqi Liu, Xiaojing Li, Jingxian Pan, Xuexue Qian, Yahui Yu, Ping Xu & Xiaofeng Xu

  2. Department of Molecular and Cell Biology, School of Life Science and Technology, Tongji University, Shanghai, 200092, China

    Bo Wang & Jian Zhu

  3. Shanghai Botanee Bio-Technology Co., Ltd., Shanghai, China

    Bo Wang

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  1. Siqi Liu
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  2. Bo Wang
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Contributions

X.X, J.Z and B.W conceived the original screening and research plans and managed the project; X.X and J.Z supervised the experiments; B.W, S.L, X.Q, J.P, X.L and Y.Y performed the experiments; S.L B.W, X.X, and J.Z wrote the manuscript and P.X reviewed and edited the manuscript.

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Correspondence to Jian Zhu or Xiaofeng Xu.

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Communicated by Wenwu Guo.

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Liu, S., Wang, B., Li, X. et al. Lateral Organ Boundaries Domain 19 (LBD19) negative regulate callus formation in Arabidopsis. Plant Cell Tiss Organ Cult 137, 485–494 (2019). https://doi.org/10.1007/s11240-019-01584-9

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  • DOI: https://doi.org/10.1007/s11240-019-01584-9

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