Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 134, Issue 2, pp 289–300 | Cite as

Promotion of shoot regeneration of Swertia chirata by biosynthesized silver nanoparticles and their involvement in ethylene interceptions and activation of antioxidant activity

  • Nirlipta Saha
  • S. Dutta Gupta
Original Article


Chemically synthesized silver nanoparticles (AgNP) have been assessed on plant tissue cultures but the impact of biologically synthesized AgNP fabricated by different phytoconstituents has not been sufficiently investigated. In this study biogenic AgNP prepared from leaf extract of Swertia chirata was utilized to address the problems associated with conservation of endangered medicinal plant through plant tissue culture. Endangered as well as economically important medicinal plant considered for this analysis was the same plant S. chirata itself. Excessive deforestation and exploration of this plant had led to complete eradication of the natural reservoir. Thus in vitro plant tissue propagation had attained much importance for maintaining the plant species. Nano-sized particles of diameter 20 nm encapped by different phytochemicals applied on regenerating shoot cultures showed enhanced shoot induction and proliferation. Other forms of silver like AgNO3 (SN) and Ag2S2O3 (STS) showed improved regeneration in comparison to control samples. Ethylene precursors like 1-aminocyclopropane-1-carboxylic acid (ACC) and 2-chloroethylphosphonic acid (CEPA) downregulated the regeneration process considerably. Reactive oxygen species (ROS) status of the treated cultures were evaluated considering hydrogen peroxide and malondialdehyde (MDA) content in treated cells as wells as their antioxidant enzyme activity. Combined manipulation and coordination of ethylene evolution, maintenance of ROS balance and better bio-acceptance of AgNP were responsible for improvement in shoot regeneration of the plant. Phytoencapsulated and nano-dimensioned Ag was capable of changing the chemical reactions of different regulating players of plant regeneration. These findings will facilitate the understanding and future utilization of biofabricated AgNP in agriculture and plant sciences.


Silver nanoparticles Plant tissue culture Shoot regeneration Ethylene Antioxidant activity 



Silver nanoparticles


Silver nitrate


Silver thiosulphate


1-Aminocyclopropane-1-carboxylic acid


2-Chloroethylphosphonic acid


Reactive oxygen species




Surface plasmon resonance


Murashige and Skoog medium




Cobalt chloride


Sodium thiosulphate


Trichloroacetic acid


Thiobarbituric acid


Superoxide dismutase






Ascorbate peroxidase


Glutathione reductase


Phenylmethylsulfonyl fluoride




Ethylene diaminetetraacetic acid


Bovine serum albumin


Nitro blue tetrazolium chloride


Oxidized glutathione


Nicotinamide adenine dinucleotide phosphate reduced


One-way analysis of variance


Duncan’s multiple range test


Least significant difference


Transmission electron microscopy


Fourier-transform infrared spectroscopy.



We gratefully acknowledge the financial support from the Department of Agriculture and Food Engineering, Indian Institute of Technology, Kharagpur.

Author contributions

All authors listed, have made considerable, direct and intellectual contribution to the work, and approved it for publication.

Compliance with ethical standards

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Supplementary material

11240_2018_1423_MOESM1_ESM.docx (18 kb)
Supplementary material 1 (DOCX 17 KB)


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© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Agriculture and Food EngineeringIndian Institute of TechnologyKharagpurIndia

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