Cotton (Gossypium hirsutum) JAZ3 and SLR1 function in jasmonate and gibberellin mediated epidermal cell differentiation and elongation
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Jasmonate ZIM-domain (JAZ) proteins and DELLA proteins are key negative regulators of jasmonates (JAs) and gibberellin (GA) signaling, respectively. In this study, we found JA and GA synergistically promote fiber cell initiation. We characterized the cellular function of a JAZ protein (GhJAZ3), and a DELLA protein (GhSLR1) of cotton (Gossypium hirsutum). GhJAZ3 is specifically expressed in elongating fibers, while GhSLR1 is expressed in different tissues and at a relatively higher level in 3 DPA ovules. GhSLR1 and GhJAZ3 proteins are localized in the cell nucleus. Yeast two-hybrid analysis indicated that GhSLR1, GhJAZ3 and GhDEL65 could interact with each other, and GhSLR1 could also interact with GhBZR1. Overexpression of GhJAZ3 in Arabidopsis increased hypocotyl and root length, leaf trichome length, and plant height, but decreased the number of leaf trichome, while overexpression of GhSLR1 in Arabidopsis decreased hypocotyl length, leaf trichome length and density. Expression of several leaf trichome initiation determinators (GL3, GL2, TTG2 and MYB23) was down-regulated in GhJAZ3 or GhSLR1 transgenic Arabidopsis, while expression of the cell elongation related genes (EXP1, EXP8, EXPL2 and XTH4) was altered in the GhJAZ3 and GhSLR1 transgenic Arabidopsis. Taken together, these results demonstrate that GhJAZ3 and GhSLR1 function in jasmonate and gibberellin mediated epidermal cell differentiation and elongation.
KeywordsGhJAZ3 GhSLR1 Jasmonate Gibberellin Epidermal cell differentiation Cell elongation
This work was supported by National Natural Sciences Foundation of China (Grant No. 31271317).
GQH, XBL, and XCX designed the experiment, analyzed the data and wrote the manuscript. XCX, and QQH performed most of the experiments. YC, WL, LHH, WYW, and MT performed some of the experiments and assisted in data analysis. All authors have read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
- Browse J (2009) Jasmonate passes muster: a receptor and targets for the defense hormone. Annu Rev Plant Biol l60:183–205. https://doi.org/10.1146/annurev.arplant.043008.092007 CrossRefGoogle Scholar
- Feng S, Martinez C, Gusmaroli G, Wang Y, Zhou J, Wang F, Chen L, Yu L, Iglesias-Pedraz JM, Kircher S, Schäfer E, Fu X, Fan LM, Deng XW (2008) Coordinated regulation of Arabidopsis thaliana development by light and gibberellins. Nature 451:475–479. https://doi.org/10.1038/nature06448 CrossRefPubMedPubMedCentralGoogle Scholar
- Ishida T, Hattori S, Sano R, Inoue K, Shirano Y, Hayashi H, Shibata D, Sato S, Kato T, Tabata S, Okada K, Wada T (2007) Arabidopsis TRANSPARENT TESTA GLABRA2 is directly regulated by R2R3 MYB transcription factors and is involved in regulation of GLABRA2 transcription in epidermal differentiation. Plant Cell 19:2531–2543CrossRefPubMedPubMedCentralGoogle Scholar
- Ishida T, Kurata T, Okada K, Wada T (2008) A genetic regulatory network in the development of trichomes and root hairs. Annu Rev Plant Biol 59:365–386. https://doi.org/10.1146/annurev.arplant.59.032607.092949 CrossRefPubMedGoogle Scholar
- Kim HJ, Hinchliffe DJ, Triplett BA, Chen ZJ, Stelly DM, Yeater KM, Moon HS, Gilbert MK, Thyssen GN, Turley RB, Fang DD (2015) Phytohormonal networks promote differentiation of fiber initials on pre-anthesis cotton ovules grown in vitro and in planta. PLoS One 10:e0125046. https://doi.org/10.1371/journal.pone.0125046 CrossRefPubMedPubMedCentralGoogle Scholar
- Qi T, Song S, Ren Q, Wu D, Huang H, Chen Y, Fan M, Peng W, Ren C, Xie D (2011) The jasmonate-ZIM-domain proteins interact with the WD-repeat/bHLH/MYB complexes to regulate jasmonate-mediated anthocyanin accumulation and trichome initiation in Arabidopsis thaliana. Plant Cell 23:1795–1814. https://doi.org/10.1105/tpc.111.083261 CrossRefPubMedPubMedCentralGoogle Scholar
- Shan CM, Shangguan XX, Zhao B, Zhang XF, Chao LM, Yang CQ, Wang LJ, Zhu HY, Zeng YD, Guo WZ, Zhou BL, Hu GJ, Guan XY, Chen ZJ, Wendel JF, Zhang TZ, Chen XY (2014) Control of cotton fibre elongation by a homeodomain transcription factor GhHOX3. Nat Commun 5:5519. https://doi.org/10.1038/ncomms6519 CrossRefPubMedPubMedCentralGoogle Scholar
- Song S, Qi T, Huang H, Ren Q, Wu D, Chang C, Peng W, Liu Y, Peng J, Xie D (2011) The jasmonate-ZIM domain proteins interact with the R2R3-MYB transcription factors MYB2 and MYB24 to affect jasmonate-regulated stamen development in Arabidopsis. Plant Cell 23:1000–1013. https://doi.org/10.1105/tpc.111.083089 CrossRefPubMedPubMedCentralGoogle Scholar
- Wang MY, Zhao PM, Cheng HQ, Han LB, Wu XM, Gao P, Wang HY, Yang CL, Zhong NQ, Zuo JR, Xia GX (2013) The cotton transcription factor TCP14 functions in auxin-mediated epidermal cell differentiation and elongation. Plant Physiol 162:1669–1680. https://doi.org/10.1104/pp.113.215673 CrossRefPubMedPubMedCentralGoogle Scholar
- Xiao YH, Li DM, Yin MH, Li XB, Zhang M, Wang YJ, Dong J, Zhao J, Luo M, Luo XY, Hou L, Hu L, Pei Y (2010) Gibberellin 20-oxidase promotes initiation and elongation of cotton fibers by regulating gibberellin synthesis. J Plant Physiol 167:829–837. https://doi.org/10.1016/j.jplph.2010.01.003 CrossRefPubMedGoogle Scholar
- Yamaguchi S (2008) Gibberellin metabolism and its regulation. Annu Rev Plant Biol 59:225–251. https://doi.org/10.1146/annurev.arplant.59.032607.092804 CrossRefPubMedGoogle Scholar
- Yang DL, Yao J, Mei CS, Tong XH, Zeng LJ, Li Q, Xiao LT, Sun TP, Li J, Deng XW, Lee CM, Thomashow MF, Yang Y, He Z, He SY (2012) Plant hormone jasmonate prioritizes defense over growth by interfering with gibberellin signaling cascade. Proc Natl Acad Sci USA 109:E1192–E1200. https://doi.org/10.1073/pnas.1201616109 CrossRefPubMedPubMedCentralGoogle Scholar
- Zhang M, Zheng X, Song S, Zeng Q, Hou L, Li D, Zhao J, Wei Y, Li X, Luo M, Xiao Y, Luo X, Zhang J, Xiang C, Pei Y (2011) Spatiotemporal manipulation of auxin biosynthesis in cotton ovule epidermal cells enhances fiber yield and quality. Nat Biotechnol 29:453–458. https://doi.org/10.1038/nbt.1843 CrossRefPubMedGoogle Scholar