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Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 133, Issue 1, pp 147–157 | Cite as

Influence of triacontanol and jasmonic acid on metabolomics during early stages of root induction in cultured tissue of tomato (Lycopersicon esculentum)

  • Malini Soundararajan
  • G. Sivakumar Swamy
  • Sumana Krishna Gaonkar
  • Sudha Deshmukh
Original Article
  • 270 Downloads

Abstract

Influence of n-triacontanol (TRIA) and jasmonic acid (JA) on metabolic profiling during root morphogenesis was studied in Lycopersicon esculentum (cv. PKM-1). Proton nuclear magnetic resonance (1H NMR) based metabolomics was employed to investigate the variations in metabolic profile. Chenomx NMR suite v.8.1 was used to identify and quantify metabolites based on their respective signature spectra. The levels of 47 metabolites were monitored for 72 h at specific time intervals (0, 3, 6, 9, 12, 24, 36, 48 and 72 h). Principal component analysis was performed to determine the variations in the metabolic profile between control and treatments during in vitro rhizogenesis. TRIA was observed to promote early root emergence (24 h) and also influence the metabolic variation during rhizogenesis between 9 and 24 h post exposure. Compounds such as IAA, ATP, NADPH, UDP-N-acetylglucosamine and gallate predominated at 9 h. Unlike TRIA, JA was unable to promote an early root induction. However, it influenced the synthesis of a relatively higher concentration of IAA at 6 h when compared to ATP, NADPH and trigonelline at 9 h. In the presence of both TRIA and JA (TRIA + JA), significant changes in the metabolic profiles were observed 24 h post exposure and the rooting was observed only after 72 h. The study suggests that TRIA may accelerate in vitro rhizogenesis of cultured tomato tissues by mainly increasing the synthesis of other growth promoting metabolites. But in the presence of JA, TRIA’s effect appears to be reduced.

Keywords

Triacontanol Jasmonic acid Rhizogenesis Metabolomics Lycopersicon esculentum Tomato 

Notes

Acknowledgements

We would like to thank Dr. Leela Iyengar (Chief Scientific Officer (Retd), I.I.T. Kanpur) for her constant support and encouragement. We acknowledge the help of the NMR facility supported by DST at Indian Institute of Science, Bangalore, where all the NMR spectra were recorded.

Author contributions

Study conception and design: GSS and SD. Acquisition of data: MS and SKG. Analysis and interpretation of data: MS. Drafting of manuscript: MS. Critical revision: GSS and SD.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  • Malini Soundararajan
    • 1
  • G. Sivakumar Swamy
    • 1
  • Sumana Krishna Gaonkar
    • 2
  • Sudha Deshmukh
    • 1
    • 3
  1. 1.Department of Biochemistry, Center for Postgraduate StudiesJain UniversityBangaloreIndia
  2. 2.NMR Research CentreIndian Institute of ScienceBangaloreIndia
  3. 3.Indian Institute of Science Education and ResearchTirupatiIndia

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