Influence of triacontanol and jasmonic acid on metabolomics during early stages of root induction in cultured tissue of tomato (Lycopersicon esculentum)
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Influence of n-triacontanol (TRIA) and jasmonic acid (JA) on metabolic profiling during root morphogenesis was studied in Lycopersicon esculentum (cv. PKM-1). Proton nuclear magnetic resonance (1H NMR) based metabolomics was employed to investigate the variations in metabolic profile. Chenomx NMR suite v.8.1 was used to identify and quantify metabolites based on their respective signature spectra. The levels of 47 metabolites were monitored for 72 h at specific time intervals (0, 3, 6, 9, 12, 24, 36, 48 and 72 h). Principal component analysis was performed to determine the variations in the metabolic profile between control and treatments during in vitro rhizogenesis. TRIA was observed to promote early root emergence (24 h) and also influence the metabolic variation during rhizogenesis between 9 and 24 h post exposure. Compounds such as IAA, ATP, NADPH, UDP-N-acetylglucosamine and gallate predominated at 9 h. Unlike TRIA, JA was unable to promote an early root induction. However, it influenced the synthesis of a relatively higher concentration of IAA at 6 h when compared to ATP, NADPH and trigonelline at 9 h. In the presence of both TRIA and JA (TRIA + JA), significant changes in the metabolic profiles were observed 24 h post exposure and the rooting was observed only after 72 h. The study suggests that TRIA may accelerate in vitro rhizogenesis of cultured tomato tissues by mainly increasing the synthesis of other growth promoting metabolites. But in the presence of JA, TRIA’s effect appears to be reduced.
KeywordsTriacontanol Jasmonic acid Rhizogenesis Metabolomics Lycopersicon esculentum Tomato
We would like to thank Dr. Leela Iyengar (Chief Scientific Officer (Retd), I.I.T. Kanpur) for her constant support and encouragement. We acknowledge the help of the NMR facility supported by DST at Indian Institute of Science, Bangalore, where all the NMR spectra were recorded.
Study conception and design: GSS and SD. Acquisition of data: MS and SKG. Analysis and interpretation of data: MS. Drafting of manuscript: MS. Critical revision: GSS and SD.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Aftab T, Khan MMA, Idrees M, Naeem M, Singh M, Ram M (2010) Stimulation of crop productivity, photosynthesis and artemisinin production in Artemisia annua L. by triacontanol and gibberellic acid application. J Plant Interact 5:273–281. https://doi.org/10.1080/17429141003647137 CrossRefGoogle Scholar
- Chen X, Yuan H, Chen R, Zhu L, Du B, Weng Q, He G (2002) Isolation and characterization of triacontanol-regulated genes in rice (Oryza sativa L.): possible role of triacontanol as a plant growth stimulator. Plant Cell Physiol 43:869–876. https://doi.org/10.1093/pcp/pcf100 CrossRefPubMedGoogle Scholar
- de Siqueira Pinto M, Abeyratne CR, Benedito VA, Peres LEP (2017) Genetic and physiological characterization of three natural allelic variations affecting the organogenic capacity in tomato (Solanum lycopersicum cv. Micro-Tom). Plant Cell Tissue Organ Cult 129:89–103. https://doi.org/10.1007/s11240-016-1159-2 CrossRefGoogle Scholar
- Dettmer J, Ursache R, Campilho A, Miyashima S, Belevich I, O’Regan S, Mullendore DL, Yadav SR, Lanz C, Beverina L, Papagni A, Schneeberger K, Weigel D, Stierhof YD, Moritz T, Knoblauch M, Jokitalo E, Helariutta Y (2014) CHOLINE TRANSPORTER-LIKE1 is required for sieve plate development to mediate long-distance cell-to-cell communication. Nat Commun 5:4276. https://doi.org/10.1038/ncomms5276 CrossRefPubMedGoogle Scholar
- Fan X, Zhang R, Cheng H (2010) Use of triacontanol in preparation of medicaments for treatment of cancers. US 7863337 B2Google Scholar
- Fliniaux O, Mesnard F, Raynaud-Le Grandic S, Baltora-Rosset S, Bienaimé C, Robins RJ, Fliniaux MA (2004) Altered nitrogen metabolism associated with de-differentiated suspension cultures derived from root cultures of Datura stramonium studied by heteronuclear multiple bond coherence (HMBC) NMR spectroscopy. J Exp Bot 55:1053–1060. https://doi.org/10.1093/jxb/erh119 CrossRefPubMedGoogle Scholar
- Georgiev MI, Radziszewska A, Neumann M, Marchev A, Alipieva K, Ludwig-Müller J (2015) Metabolic alterations of Verbascum nigrum L. plants and SAArT transformed roots as revealed by NMR-based metabolomics. Plant Cell Tissue Organ Cult 123:349–356. https://doi.org/10.1007/s11240-015-0840-1 CrossRefGoogle Scholar
- Gong B, Wang X, Wei M, Yang F, Li Y, Shi Q (2016) Overexpression of S-adenosylmethionine synthetase 1 enhances tomato callus tolerance to alkali stress through polyamine and hydrogen peroxide cross-linked networks. Plant Cell Tissue Organ Cult 124:377–391. https://doi.org/10.1007/s11240-015-0901-5 CrossRefGoogle Scholar
- Khandaker MM, Faruq G, Rahman MM, Sofian-Azirun M, Boyce AN (2013) The influence of 1-triacontanol on the growth, flowering, and quality of potted bougainvillea plants (Bougainvillea glabra var. “Elizabeth Angus”) under natural conditions. Sci World J. https://doi.org/10.1155/2013/308651 Google Scholar
- Mira MM, Wally OSD, Elhiti M, El-Shanshory A, Reddy DS, Hill RD, Stasolla C (2016) Jasmonic acid is a downstream component in the modulation of somatic embryogenesis by Arabidopsis class 2 phytoglobin. J Exp Bot 67:2231–2246. https://doi.org/10.1093/jxb/erw022 CrossRefPubMedPubMedCentralGoogle Scholar
- Palama TL, Menard P, Fock I, Choi YH, Bourdon E, Govinden-Soulange J, Bahut M, Payet B, Verpoorte R, Kodja H (2010) Shoot differentiation from protocorm callus cultures of Vanilla planifolia (Orchidaceae): proteomic and metabolic responses at early stage. BMC Plant Biol 10:82. https://doi.org/10.1186/1471-2229-10-82 CrossRefPubMedPubMedCentralGoogle Scholar
- Ravnikar M, Rode J, Gogala N, Benedicic D (1990) Regulation of organogenesis with jasmonic acid. Acta Hort 169–172. https://doi.org/10.17660/ActaHortic.1990.280.29
- Ries SK, Wert VF (1982) Rapid in vivo and in vitro effects of triacontanol. J Plant Growth Regul 1:117–127Google Scholar
- Saiman MZ, Mustafa NR, Choi YH, Verpoorte R, Schulte AE (2015) Metabolic alterations and distribution of five-carbon precursors in jasmonic acid-elicited Catharanthus roseus cell suspension cultures. Plant Cell Tissue Organ Cult 122:351–362. https://doi.org/10.1007/s11240-015-0773-8 CrossRefGoogle Scholar
- Shah SH, Ali S, Jan SA, Jalal-Ud-Din, Ali GM (2015) Piercing and incubation method of in planta transformation producing stable transgenic plants by overexpressing DREB1A gene in tomato (Solanum lycopersicum Mill.). Plant Cell Tissue Organ Cult 120:1139–1157. https://doi.org/10.1007/s11240-014-0670-6 CrossRefGoogle Scholar
- Smith AM, Coupland G, Dolan L, Harberd N, Jones J, Martin C, Sablowski R, Amey A (2009) Plant biology. Garland Science, New YorkGoogle Scholar
- Swamy SG, Ramanarayan K, Inamdar LS, Inamdar SR (2009) Triacontanol and jasmonic acid differentially modulate the lipid organization as evidenced by the fluorescent probe behavior and 31P nuclear magnetic resonance shifts in model membranes. J Membr Biol 228:165–177. https://doi.org/10.1007/s00232-009-9198-9 CrossRefPubMedGoogle Scholar
- Yaseen M, Tajuddin K (1998) Effect of plant growth regulators on yield, oil composition and artemisinin of Artemisia annua under temperate conditions. J Med Aromat Plant Sci 20:1038–1041Google Scholar
- Zaragoza-Martínez F, Lucho-Constantino GG, Ponce-Noyola T, Esparza-García F, Poggi-Varaldo H, Cerda-García-Rojas CM, Trejo-Tapia G, Ramos-Valdivia AC (2016) Jasmonic acid stimulates the oxidative responses and triterpene production in Jatropha curcas cell suspension cultures through mevalonate as biosynthetic precursor. Plant Cell Tissue Organ Cult 127:47. https://doi.org/10.1007/s11240-016-1028-z CrossRefGoogle Scholar
- Zulak KG, Weljie AM, Vogel HJ, Facchini PJ (2008) Quantitative 1H NMR metabolomics reveals extensive metabolic reprogramming of primary and secondary metabolism in elicitor-treated opium poppy cell cultures. BMC Plant Biol 8:5. https://doi.org/10.1186/1471-2229-8-5 CrossRefPubMedPubMedCentralGoogle Scholar