A homologue of the Arabidopsis gene FUSCA3 (FUS3), isolated from the protocorm-like body (PLB) of Rosa canina and designated RcFUS3, encodes 331 amino acid residues. It was shown that RcFUS3 is specifically expressed in the PLB of R. canina and that its subcellular localization is in the nucleus. The Arabidopsis fus3-3 mutant phenotype could be rescued by over-expression of RcFUS3, suggesting that RcFUS3 has a function similar to that of Arabidopsis FUS3. Over-expression of RcFUS3 in wild type Arabidopsis resulted in a decrease in endogenous GA and CTK levels, an increase in ABA and IAA contents, starch grain accumulation in the cotyledon and hypocotyl, failure of cotyledon extension and abnormal elongation of the hypocotyl, abnormal stomatal and pavement cells, an increase in branch numbers, prolongation of the growth cycle, and morphological changes in floral organs. Interestingly, over-expression of RcFUS3 in homozygous form resulted in premature degradation of the tapetum, indehiscent anthers and hypogenetic stamens, causing complete male sterility in Arabidopsis; this is the first observation that over-expression of a gene (RcFUS3) homologous to FUS3 can lead to male sterility and starch grain accumulation in Arabidopsis.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
Tax calculation will be finalised during checkout.
- GA3 :
Green fluorescent protein
Murashige and Skoog
Open reading frame
Rapid amplification of cDNA ends
Reverse transcription polymerase chain reaction
Scanning electron microscopy
Baumbusch LO, Hughes DW, Galau GA, Jakobsen KS (2004) LEC1, FUS3, ABI3 and Em expression reveals no correlation with dormancy in Arabidopsis. J Exp Bot 55(394):77–87
Castle LA, Meinke DW (1994) A FUSCA gene of Arabidopsis encodes a nove1 protein essential for plant development. Plant Cell 6:25–41
Cheng H, Qin L, Lee S, Fu X, Richards DE, Cao D, Luo D, Harberd NP, Peng J (2004) Gibberellin regulates Arabidopsis floral development via suppression of DELLA protein function. Development 131:1055–1064
Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16(6):735–743
Cui Y, Liu K, Xu C, Liu F, Li QX, Liu S, Wang B (2014) Development of a sensitive monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for analysing chlorantraniliprole residues. Food Chem 143:293–299
Curaba J, Moritz T, Blervaque R, Parcy F, Raz V, Herzog M, Vachon G (2004) AtGA3ox2, a key gene responsible for bioactive gibberellin biosynthesis, is regulated during embryogenesis by LEAFY COTYLEDON2 and FUSCA3 in Arabidopsis. Plant Physiol 136:3660–3669
Dekkers BJ, Schuurmans JA, Smeekens SC (2004) Glucose delays seed germination in Arabidopsis thaliana. Planta 218(4):579–588
Fujihira K, Kurata T, Watahiki MK, Karahara I, Yamamoto KT (2000) An agravitropic mutant of Arabidopsis, endodermal-amyloplast less 1, that lacks amyloplasts in hypocotyl endodermal cell layer. Plant Cell Physiol 41(11):1193–1199
Fukaki H, Wysocka-Diller J, Kato T, Fujisawa H, Benfey PN, Tasaka M (1998) Genetic evidence that the endodermis is essential for shoot gravitropism in Arabidopsis thaliana. Plant J 14:425–430
Gazzarrini S, Tsuchiya Y, Lumba S, Okamoto M, McCourt P (2004) The transcription factor FUSCA3 controls developmental timing in Arabidopsis through the hormones gibberellin and abscisic acid. Develop Cell 7:373–385
Goto N, Pharris RP (1999) Role of gibberellins in the development of floral organs of the gibberellin-deficient mutant ga1-1 of Arabidopsis thaliana. Can J Bot 77:944–954
Jacobsen SE, Olszewski NE (1991) Characterization of the arrest in anther development associated with gibberellin deficiency of the gib-1 mutant of tomato. Plant Physiol 97:409–414
Luerssen H, Kirik V, Herrmann P, Miséra S (1998) FUSCA3 encodes a protein with a conserved VP1/ABI3-like B3 domain which is of functional importance for the regulation of seed maturation in Arabidopsis thaliana. Plant J 15(6):755–764
Lumba S, McCourt P (2005) Preventing leaf identity theft with hormones. Curr Opin Plant Biol 8:501–505
Müller AJ (1963) Embryonentest zum Nachweis rezessiver Letalfaktoren bei Arabidopsis thaliana. Biol Zbl 82:133–163
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15(3):473–497
Reidt W, Wohlfarth T, Ellerström M, Czihal A, Tewes A, Ezcurra I, Rask L, Bäumlein H (2000) Gene regulation during late embryogenesis: the RY motif of maturation-specific gene promoters is a direct target of the FUS3 gene product. Plant J 21:401–408
Roscoe TT, Guilleminot J, Bessoule JJ, Berger F, Devic M (2015) Complementation of seed maturation phenotypes by ectopic expression of ABSCISIC ACID INSENSITIVE3, FUSCA3 and LEAFY COTYLEDON2 in Arabidopsis. Plant Cell Physiol 56:1215–1228
Saibo NJ, Vriezen WH, Beemster GT, Van Der Straeten D (2003) Growth and stomata development of Arabidopsis hypocotyls are controlled by gibberellins and modulated by ethylene and auxins. Plant J 33:989–1000
Srinivasan A, Yamini KN, Reddy SS, Kumar VD (2015) Tapetum specific expression of unedited nad3 gene from safflower and targeting the protein into mitochondria, induces male sterility in transgenic tobacco plants. Plant Cell Tiss Organ Cult 120:387–398
Tang LP, Zhou C, Wang SS, Yuan J, Zhang XS, Su YH (2016) FUSCA3 interacting with LEAFY COTYLEDON2 controls lateral root formation through regulating YUCCA4 gene expression in Arabidopsis thaliana. New Phytol. https://doi.org/10.1111/nph.14313
Tian C, Chen Y, Zhao X, Zhao L (2008) Plant regeneration through protocorm-like bodies induced from rhizoids using leaf explants of Rosa spp. Plant Cell Rep 27(5):823–831
Wang B, He Z, Zhao J (1998) Study on Bt toxin ELISA for transgenic plant. Acta Gossypii Sinica 10:27–30
Wang H, Guo J, Lambert KN, Lin Y (2007) Developmental control of Arabidopsis seed oil biosynthesis. Planta 226:773–783
Xu K, Chang Y, Liu K, Wang F, Liu Z, Zhang T, Li T, Zhang Y, Zhang F, Zhang J, Wang Y, Niu W, Jia S, Xie H, Tan G, Li C (2014a) Regeneration of Solanum nigrum by somatic embryogenesis, involving frog egg-like body, a novel structure. PLoS ONE 9(6):e98672
Xu K, Huang X, Wu M, Wang Y, Chang Y, Liu K, Zhang J, Zhang Y, Zhang F, Yi L, Li T, Wang R, Tan G, Li C (2014b) A rapid, highly efficient and economical method of Agrobacterium-mediated in planta transient transformation in living onion epidermis. PLoS ONE 9(1):e83556
Zhang M, Cao X, Jia Q, Ohlrogge J (2016) FUSCA3 activates triacylglycerol accumulation in Arabidopsis seedlings and tobacco BY2 cells. Plant J. https://doi.org/10.1111/tpj.13233
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Communicated by Sergio J. Ochatt.
About this article
Cite this article
Xu, K., Zhang, J., Wu, J. et al. Overexpression of RcFUSCA3, a B3 transcription factor from the PLB in Rosa canina, activates starch accumulation and induces male sterility in Arabidopsis. Plant Cell Tiss Organ Cult 133, 87–101 (2018). https://doi.org/10.1007/s11240-017-1364-7
- Protocorm-like body
- Rosa canina
- Male sterility
- Starch accumulation