Abstract
Plant genetic transformation and regeneration has become a valuable research tool for functional genomics. A successful transformation event involves the transfer of the target gene into a suitable explant, the integration and expression of the transgene into the host genome and the regeneration of the fertile transgenic plants from the transformed tissues. Wheat is considered as a recalcitrant species even if many efforts have been done in recent years to improve transformation efficiency. The transformation of its progenitors has never been attempted, even though the possibility to transform wild hemmer represents a valuable tool to evaluate structural and functional variability occurring in wild hemmer and explaining its higher adaptation to abiotic stresses. In this paper we report, as far as we know, for the first time, the microparticle transformation of immature embryos of the wild hemmer Triticum dicoccoides with the Tapgip1 gene. The transformation method was successfully transferred from durum wheat and several transgenic lines were obtained. Its application for the exploitation of wheat progenitors for molecular breeding is of great relevance for genomic and functional genomics studies. This result, indeed, opens new perspectives in complementation studies for the comprehension of durum and bread wheat adaptation mechanisms to stresses.
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MJ, SM and RD wrote the manuscript; MJ, TB, MDG, IM, RL, AG, CV performed the experiments and helped in the tissue culture. RD coordinated the work. All authors read and approved the final manuscript.
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Communicated by Jannusz Zimmny.
In memory of Renato D’Ovidio.
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Janni, M., Bozzini, T., Di Giovanni, M. et al. First production of wild hemmer (Triticum turgidum ssp. dicoccoides) transgenic plants. Plant Cell Tiss Organ Cult 132, 461–467 (2018). https://doi.org/10.1007/s11240-017-1342-0
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DOI: https://doi.org/10.1007/s11240-017-1342-0