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Rice inositol polyphosphate kinase gene (OsIPK2), a putative new player of gibberellic acid signaling, involves in modulation of shoot elongation and fertility

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Abstract

Gibberellic acid (GA) is an important plant hormone mediating plant growth and development throughout the life span. Although many GA biosynthesis genes and signaling components have been revealed, the signal transduction mechanisms from GA perception to physiological actions are still largely unclear. In this study, we investigated the functions of a rice (Oryza sativa) inositol polyphosphate kinase gene (OsIPK2) in rice growth and development, showing that OsIPK2 is a putative new player in GA signaling. OsIPK2 is widely expressed in rice with high accumulation in tender and rapidly dividing tissues. The OsIPK2 protein is mainly localized in the nucleus and plasma membrane. To study the biological roles of OsIPK2 in rice, RNA interference and overexpression transgenic plants were generated. OsIPK2 antisense plants exhibited taller seedling height and lower fertility rate than the wild type, while overexpression lines showed reduced plant height. Microarray and qRT-PCR assays showed that expression levels of several GA-related genes were altered in transgenic plants. Besides, down-regulation of OsIPK2 resulted in hypersensitivity to paclobutrazol (PAC), a GA biosynthesis inhibitor. We also described that the expression of OsIPK2 could be either induced by GA or repressed by PAC. Taken together, these findings suggested that OsIPK2 is likely a negative regulator of GA signaling and involves in modulating shoot elongation and fertility.

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Acknowledgements

We sincerely thank Prof. Huijun Xia (Wuhan University) for providing helpful advice and experimental conditions; State Key Laboratory of Hybrid Rice for reagents and analytical equipments; Prof. Yongjun Lin (Huazhong Agricultural University) and Yue Li for rice transformation work; Prof. Yuqi Feng (Wuhan University) for quantification of the endogenous ABA content; Prof. Zheng Meng (Institute of Botany, the Chinese Academy of Sciences) for support the pBJWI3 and PU1301 vectors; Prof. Lizhong Xiong (Huazhong Agricultural University) for support the pU1391cGFP vector. We also thank Dr. Xiaolei Fan for northern blot hybridization experiments. This work was supported by the National High Technology Research and Development Program of China (Grant 2006AA10A103).

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  1. State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, Hubei, China

    Yao Chen, Zhaoyun Wei, Qiaofeng Yang, Sihong Sang & Peng Wang

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  1. Yao Chen
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  2. Zhaoyun Wei
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  3. Qiaofeng Yang
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  4. Sihong Sang
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  5. Peng Wang
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YC conceived and designed research. YC, ZW, QY and SS conducted experiments. YC analyzed data and wrote the manuscript.

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Correspondence to Yao Chen.

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Communicated by Ming-Tsair Chan.

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Chen, Y., Wei, Z., Yang, Q. et al. Rice inositol polyphosphate kinase gene (OsIPK2), a putative new player of gibberellic acid signaling, involves in modulation of shoot elongation and fertility. Plant Cell Tiss Organ Cult 131, 471–482 (2017). https://doi.org/10.1007/s11240-017-1298-0

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