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Production of sapogenins (stigmasterol and hecogenin) from genetically transformed hairy root cultures of Chlorophytum borivilianum (Safed musli)

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Abstract

Chlorophytum borivilianum belonging to the family Liliaceae, is distributed in the pantropical regions of India and South Africa. The sapogenins (stigmasterol and hecogenin) of C. borivilianum are well known for their appetizing and aphrodisiac properties. The present study involves enhancing the sapogenin content in C. borivilianum by genetic transformations with Agrobacterium rhizogenes strains (MTCC 2364 and 532, PRT Gus). A maximum transformation frequency of 98% was obtained with Agrobacterium rhizogenes MTCC 2364 strain with rhizome explants after a co-cultivation period of 48 h. Two potential rhizoclones (2364a and 2364b) were selected for the production of stigmasterol and hecogenin. The maximum production of stigmasterol (83.952 ± 0.01 mg/g) was seen in 2364b rhizoclone, whereas, the highest accumulation of hecogenin (81.52 ± 0.02 mg/g) was observed in 2364a rhizoclone. The C. borivilianum hairy root cultures obtained in this study provide a continuous and sustainable production of stigmasterol and hecogenin on a commercial scale.

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Acknowledgements

This research work has been supported by JNTUH, Kukatpally. We sincerely thank them for their support.

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Authors and Affiliations

  1. Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Hyderabad, India

    Gayathri Bathoju, Kiranmayee Rao & Archana Giri

Authors
  1. Gayathri Bathoju
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  2. Kiranmayee Rao
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  3. Archana Giri
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Contributions

GB has done the entire work, KR helped in paper writing, AG was the guide in Research.

Corresponding author

Correspondence to Archana Giri.

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There is no conflict of interest between the authors.

Additional information

Communicated by Dr. Silvia Moreno.

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Bathoju, G., Rao, K. & Giri, A. Production of sapogenins (stigmasterol and hecogenin) from genetically transformed hairy root cultures of Chlorophytum borivilianum (Safed musli). Plant Cell Tiss Organ Cult 131, 369–376 (2017). https://doi.org/10.1007/s11240-017-1290-8

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  • DOI: https://doi.org/10.1007/s11240-017-1290-8

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