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Evaluation of haploidization efficiency in winter squash (Cucurbita maxima Duch.) and pumpkin (Cucurbita moschata Duch.) through anther culture

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Abstract

The production of large numbers of haploids is the crucial point of the dihaploidisation process. Although in vitro haploid plants were successfully produced by irradiated pollen technique in winter squash (Cucurbita maxima Duch.) and pumpkin (Cucurbita moschata Duch.), the frequency is still insufficient for using in a large-scale breeding programme. Thus, the present study was conducted to determine the efficacy of the anther culture technique on the production of in vitro haploids in the aforementioned species for which there have been no successful reports concerning by androgenesis. The anthers at uninucleate microspore stage were collected at different florescence times and then cultured on a solid MS medium supplemented by different combinations of 2,4-D (2,4-dichlorophenoxyacetic acid), BAP (6-benzylaminopurine), KN (kinetin) with the constant addition of NAA (naphthalene acetic acid) to induce callogenesis, embryogenesis and plantlet initiation. The combination of PGR, genotype and anther collection time played an important role in the androgenic response. The highest response was obtained from 57Sİ21 and G9 lines with the combination of 2.0 or 4.0 mg/l BAP + 0.05 mg/l NAA (E6 medium) at the first anther collection time. Plantlets were regenerated and rooted on MS medium supplemented by 0.01 mg/l IAA. In total, 74 plants were recovered and propagated with micro-cuttings. The ploidy analyses revealed that 35 plants (47.3 %) were haploid (n = 20), and the others (52.7 %) were diploid (2n = 40).

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Acknowledgments

We gratefully acknowledge the support of the Ondokuz Mayis University (Project No: PYO.BMY.1901.10.002), Samsun, Turkey.

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Authors and Affiliations

  1. Vocational High School of Bafra, Ondokuz Mayis University, Tekel Street, 55400, Samsun, Turkey

    Ertan Sait Kurtar

  2. Horticulture Department of Agriculture Faculty, Ondokuz Mayis University, Samsun, Turkey

    Ahmet Balkaya

  3. Vocational High School of Samsun, Ondokuz Mayis University, Samsun, Turkey

    Dilek Kandemir

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  1. Ertan Sait Kurtar
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  2. Ahmet Balkaya
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Correspondence to Ertan Sait Kurtar.

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Kurtar, E.S., Balkaya, A. & Kandemir, D. Evaluation of haploidization efficiency in winter squash (Cucurbita maxima Duch.) and pumpkin (Cucurbita moschata Duch.) through anther culture. Plant Cell Tiss Organ Cult 127, 497–511 (2016). https://doi.org/10.1007/s11240-016-1074-6

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