Skip to main content
SpringerLink
Log in

Effects of ethylene on shoot initiation, leaf yellowing, and shoot tip necrosis in roses

Plant Cell, Tissue and Organ Culture (PCTOC) Aims and scope Submit manuscript

Cite this article

Abstract

This study was conducted to investigate the in vitro influence of ethylene on shoot branching and leaf yellowing in the rose cultivar Tineke by using different compounds that regulate ethylene inhibition and stimulation. Aminoethoxy vinyl glycine (AVG), silver thiosulfate (STS), and sodium nitroprusside (SNP) caused enhanced apical shoot initiation and reduced leaf yellowing, via inhibition of ethylene production, in the following order: AVG > SNP > STS. In contrast, the addition of 1-aminocyclopropane-1-carboxylic acid (ACC) or 3-indoleacetic acid (IAA) stimulated ethylene production and had greater negative effects on the studied parameters than the control; the negative effects of IAA were further confirmed in combination with AVG, STS, or SNP. The effects of ethylene on apical shoot initiation and leaf yellowing in Tineke were confirmed in another rose cultivar, Innocence. Hence, this study provides strong support for the hypothesis that ethylene-inhibiting agents have beneficial effects on apical shoot initiation and reduction of leaf yellowing in other rose cultivars.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Bogaert I, Van Cauter S, Prinsen E, Doležal K, Werbrouck S (2006) New aromatic cytokinin can make the difference. Acta Hortic 725:265–270

  • Burgos L, Alburquerque N (2003) Low kanamycin concentration and ethylene inhibitors improve adventitious regeneration from apricot leaves. Plant Cell Rep 21:1167–1174

    Article  CAS  PubMed  Google Scholar 

  • Chae SC, Kim HH, Park SU (2012) Ethylene inhibitors enhance shoots organogenesis of gloxinia (Sinningia speciosa). Sci World J 2012:859381

    Article  Google Scholar 

  • Dubois LAM, de Vries DP (1995) Prolongation of vase life of cut roses via introduction of genes coding for antibacterial activity, somatic embryogenesis and Agrobacterium mediated transformation. Acta Hort 405:205–209

    Google Scholar 

  • Gaspar T, Kevers C, Bouillenne H, Maziere Y, Barbe JP (1989) Ethylene production in relation to rose micropropagation through axillary budding. In: Clijsters M (ed) De proft physiological aspects of ethylene production in lower and higher plants, (pp 303–312)

  • Horn WAH (1992) Micropropagation of rose (Rosa L). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry vol 20 high-tech and micropropagation IV. Germany, Springer, pp 320–342

    Google Scholar 

  • Hsia C, Korban S (1996) Organogenesis and somatic embryogenesis in callus cultures of Rosa hybrida and Rosa chinensis minima. Plant Cell Tissue Organ Cult 44:1–6

    Article  CAS  Google Scholar 

  • Kevers C, Boyer N, Courduroux JC, Gasper T (1992) The influence of ethylene on proliferation and growth of rose shoot culture. Plant Cell Tissue Organ Cult 28:175–181

    Article  CAS  Google Scholar 

  • Kumar S, Dudley J (2007) Bioinformatics for biologists in the genomics era. Bioinformatics. doi:10.1093/bioinformatics/btm239

    Google Scholar 

  • Martinez MT, Vieitez AM, Corredoira E (2015) Improved secondary embryo production in Quercus alba and Quercus rubra by activated charcoal, silver thiosulphate and sucrose: influence of embryogenic explant used for subculture. Plant Cell Tissue Organ Cult 121:531–546

    Article  CAS  Google Scholar 

  • Mekers O, Meiresonne L, Meneve I (1984) Ethylene production inhibitors can improve in vitro propagation of roses. Med Fac Landbouww Rijksuniv Gent 49/3b:1144–1939

    Google Scholar 

  • Murashige I, Skoog F (1962) A revised medium for rapid growth and bioassys with tobacco tissue culture. Plant Physiol 15:473–497

  • Naing AH, Min JS, Han JS, Lim SH, Lim KB, Kim CK (2014) Factors influencing in vitro shoot regeneration from leaf segments of Chrysanthemum. C R Biol 337:383–390

    Article  PubMed  Google Scholar 

  • Naing AH, Park KI, Chung MY, Lim KB, Kim CK (2015) Optimization of factors affecting efficient shoot regeneration in Chrysanthemum cv. Shinma. Braz J Bot. doi:10.1007/s40415-015-0143-0

    Google Scholar 

  • Pati PK, Sharma M, Sood A, Ahuja PS (2004) Direct shoot regeneration from leaf explants of R. damascena Mill. In Vitro Cell Dev Biol Plant 40:192–195

    Article  Google Scholar 

  • Pati PK, Rath SP, Sharma M, Sood A, Ahuja PS (2006) In vitro propagation of rose—a review. Biotechnol Adv 24:94–114

    Article  CAS  PubMed  Google Scholar 

  • Podwyszyńska M, Goszczyńska D (1998) Effect of inhibitors of ethylene biosynthesis and action, as well as calcium and magnesium on rose shoot rooting, shoot-tip necrosis and leaf senescence in vitro. Acta Physiol Plant 20:91–98

    Article  Google Scholar 

  • Reid MS, Paull JL, Farhoomand MB, Kofranek AM, Staby GL (1980) Pulse treatments with the silver thiosulphate complex extend the vase life of cut carnations. J Am Soc Hort Sci 105:25–27

    CAS  Google Scholar 

  • Rosu A, Skirvin RM, Bein A, Norton MA, Kushad M (1995) The development of putative adventitious shoots from a chimeral thornless rose (Rosa multiflora thunb. ex J. Murr.) in vitro. J Hort Sci 70:901–907

    Google Scholar 

  • Schum A, Hofmann K, Ghalib N, Tawfik A (2001) Factors affecting protoplast isolation and plant regeneration in Rosa spp. Gartenbauwiss 66:115–122

    CAS  Google Scholar 

  • Sgamma T, Thomas B, Muleo R (2015) Ethylene inhibitor silver nitrate enhances regeneration and genetic transformation of Prunus avium (L.) cv Stella. Plant Cell Tissue Organ Cult 120:79–88

    Article  CAS  Google Scholar 

  • Songstad DD, Duncan DR, Widholm JM (1988) Effect of 1-aminocycopropane-1-carboxilic acid, silver nitrate, and norbornadiene on plant regeneration from maize callus cultures. Plant Cell Rep 7:262-265

    Article  Google Scholar 

  • Tian C, Chen Y, Zhao X, Zhao L (2008) Plant regeneration through protocorm-like bodies induced from rhizoids using leaf explants of Rosa spp. Plant Cell Rep 27:823–831

    Article  CAS  PubMed  Google Scholar 

  • Widholm JM (1988) Effect of 1-aminocycopropane-1-carboxilic acid, silver nitrate, and norbornadiene on plant regeneration from maize callus cultures. Plant Cell Rep 7:262–265

    Article  PubMed  Google Scholar 

  • Würschum T, Tucker MR, Maurer HP, Leiser WL (2015) Ethylene inhibitors improve efficiency of microspore embryogenesis in hexaploid triticale. Plant Cell Tissue Organ Cult 122:751–757

    Article  Google Scholar 

  • Xu J, Yin H, Wang W, Mi Q, Liu X (2009) Effects of sodium nitroprusside on callus induction and shoot regeneration in micropropagated Dioscorea opposite. Plant Growth Regul 59:279–285

    Article  CAS  Google Scholar 

  • Zhu S, Zhou J (2007) Effect of nitric oxide on ethylene production in strawberry fruit during storage. Food Chem 100:1517–1522

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

    Authors and Affiliations

    1. Department of Horticultural Science, Kyungpook National University, Daegu, 702-701, South Korea

      Jun Seong Park, Aung Htay Naing & Chang Kil Kim

    Authors
    1. Jun Seong Park
      View author publications

      You can also search for this author in PubMed Google Scholar

    2. Aung Htay Naing
      View author publications

      You can also search for this author in PubMed Google Scholar

    3. Chang Kil Kim
      View author publications

      You can also search for this author in PubMed Google Scholar

    Corresponding author

    Correspondence to Chang Kil Kim.

    Additional information

    Aung Htay Naing and Jun Seong Park contributed equally as first authors.

    Rights and permissions

    Reprints and Permissions

    About this article

    Check for updates. Verify currency and authenticity via CrossMark

    Cite this article

    Park, J.S., Naing, A.H. & Kim, C.K. Effects of ethylene on shoot initiation, leaf yellowing, and shoot tip necrosis in roses. Plant Cell Tiss Organ Cult 127, 425–431 (2016). https://doi.org/10.1007/s11240-016-1066-6

    Download citation

    • Received:

    • Accepted:

    • Published:

    • Issue Date:

    • DOI: https://doi.org/10.1007/s11240-016-1066-6

    Keywords

    search

    Navigation