ZmCIPK8, a CBL-interacting protein kinase, regulates maize response to drought stress
Plant CBL-interacting protein kinases (CIPKs) play an important role in stress signaling transduction and enhancing plant stress tolerance. However, the functions of most CIPKs in crop plants such as maize have not been studied. Here, a novel CIPK gene, ZmCIPK8, was cloned and characterized from maize (Zea mays). The ZmCIPK8 gene has 14 introns and its encoded protein shares high homology to Arabidopsis and rice CIPKs. Yeast two-hybrid and bimolecular fluorescence complementation assay demonstrated that ZmCIPK8 interacted with ZmCBL1, ZmCBL4 and ZmCBL9. Quantitative RT-PCR analysis revealed that the mRNA accumulation of ZmCIPK8 in maize leaves and roots was promoted by drought stress. GUS gene expression driven by the ZmCIPK8 promoter was in an organ-dependent pattern and induced in Arabidopsis seedlings under drought stress. Over-expression of ZmCIPK8 in tobacco induced the expression of the NAC, CBF, and Rd29A genes and enhanced drought tolerance of transgenic tobacco seedlings. Thus, ZmCIPK8 perhaps is involved in plant response to drought and other abiotic stress through regulating stress-related genes.
KeywordsMaize CIPK Drought stress Transgenic plants
This work was supported by National Natural Science Foundation of China (http://www.nsfc.gov.cn/; grant no. 31100200, to FJT), Plan for Scientific Innovation Talent of Henan Province (http://www.hnkjt.gov.cn/; grant no. 144200510012, to WW), Key Project of Henan Educational Committee (http://www.haedu.gov.cn/; grant no. 14A180005, to FJT) and State Key Laboratory of Crop Biology in Shandong Agricultural University (http://klab.sdau.edu.cn/; grant no. 2014KF04, to FJT).
- D’Angelo C, Weinl S, Batistic O, Pandey GK, Cheong YH, Schültke S, Albrecht V, Ehlert B, Schulz B, Harter K, Luan S, Bock R, Kudla J (2006) Alternative complex formation of the Ca-regulated protein kinase CIPK1 controls abscisic acid-dependent and independent stress responses in Arabidopsis. Plant J 48:857–872CrossRefPubMedGoogle Scholar
- Hu L, Hu T, Zhang X, Pang H, Fu J (2012) Exogenous glycine betaine ameliorates the adverse effect of salt stress on perennial ryegrass. J Am Soc Hortic Sci 137:38–46Google Scholar
- Liu LL, Ren HM, Chen LQ, Wang Y, Wu WH (2013) A protein kinase, calcineurin B-like protein-interacting protein kinase 9, interacts with calcium sensor calcineurin B-like protein 3 and regulates potassium homeostasis under low-potassium stress in Arabidopsis. Plant Physiol 161:266–277PubMedCentralCrossRefPubMedGoogle Scholar
- Ludwig AA, Saitoh H, Felix G, Freymark G, Miersch O, Wasternack C, Boller T, Jones JD, Romeis T (2005) Ethylene-mediated cross-talk between calcium-dependent protein kinase and MAPK signaling controls stress responses in plants. Proc Natl Acad Sci USA 102:10736–10741PubMedCentralCrossRefPubMedGoogle Scholar
- Man D, Bao YX, Han LB, Zhang X (2011) Drought tolerance associated with proline and hormone metabolism in two tall fescue cultivars. Hortic Sci 46:1027–1032Google Scholar
- Piao HL, Xuan YH, Park SH, Je BI, Park SJ, Kim CM, Huang J, Wang GK, Kim MJ, Kang SM, Lee IJ, Kwon TR, Kim YH, Yeo US, Yi G, Son D, Han CD (2010) OsCIPK31, a CBL-interacting protein kinase is involved in germination and seedling growth under abiotic stress conditions in rice plants. Mol Cell 30:19–27CrossRefGoogle Scholar