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Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 123, Issue 3, pp 443–453 | Cite as

Elicitation of furanocoumarins in poison hemlock (Conium maculatum L.) cell culture

  • Philipp Meier
  • Hannu Hotti
  • Heiko RischerEmail author
Original Article

Abstract

Furanocoumarins, such as psoralen, xanthotoxin and bergapten, serve as protectants against phytopathogens and are used in pharmaceutical applications e.g. as DNA-crosslinking agents against non-melanoma skin cancers. Poison hemlock plants (Conium maculatum L.) are a known source of furanocoumarins and toxic alkaloids, but systematic research on callus and suspension cultures with the aim of eliciting secondary metabolites is lacking. Therefore callus cultures of poison hemlock were induced with 0.186 mg L−1 6-benzylaminopurine and 2 or 4 mg L−1 naphthalene acetic acid on McCown’s Woody plant medium. A broad variety of elicitors (alginic acid, cellulase, chitosan, ethylene, methyl jasmonate, salicylic acid, copper(II) sulphate and silver nitrate) were tested with an established cell suspension culture for their capacity to trigger differential metabolite accumulation. Samples were extracted and analysed by gas chromatography-mass spectrometry. Elicitation with alginic acid, cellulase, chitosan, silver nitrate and copper(II) sulphate induced furanocoumarins. Plant hormones (ethylene, methyl jasmonate and salicylic acid) were not able to induce furanocoumarins. Extracts contained bergapten, columbianetin, isopimpinellin, marmesin, oroselone, psoralen and xanthotoxin but not piperidine alkaloids. The relative amount of furanocoumarins was generally higher in the medium than in the cells. The report describes the angular furanocoumarins oroselone and columbianetin together with the linear furanocoumarin marmesin, elicited for the first time in poison hemlock.

Keywords

Callus Cell culture Elicitation Furanocoumarins Poison hemlock (Conium maculatum L.) 

Abbreviations

BA

6-Benzylaminopurine

DCW

Dry cell weight

FCW

Fresh cell weight

FDA

Fluorescein diacetic acid

GC-MS

Gas chromatography mass spectrometry

NAA

Naphthalene acetic acid

Rt

Retention time

TLC

Thin layer chromatography

WP

McCown’s Woody plant medium (Lloyd and McCown 1981)

Notes

Acknowledgments

We thank Anna Ruskeepää and Tuulikki Seppänen-Laakso for GC-MS analyses. Prof. Dr. Regine Eibl-Schindler, Zurich University of Applied Sciences, is acknowledged for enabling P.M.’s bachelor thesis at VTT. This work was supported by ERASMUS funding (to P.M.), the Finnish Doctoral Program in Plant Science, Societas pro Fauna et Flora Fennica, Eteläsuomalaisten ylioppilaiden säätiö, Oskar Öflunds Stiftelse, the Otto A. Malm Foundation (all to H.H.), VTT and the Academy of Finland (grant 138808 to H.R.). The authors acknowledge the support of COST Action FA1006, PlantEngine.

Author contributions

P.M., H.H., H.R. planned the experiments; P.M., H.H. performed the experiments; P.M., H.H., H.R. analysed the data; P.M., H.H., H.R. wrote the paper.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11240_2015_847_MOESM1_ESM.doc (71 kb)
Supplementary material 1 (DOC 71 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.Life Sciences und Facility ManagementZHAW Zurich University of Applied SciencesWaedenswilSwitzerland
  2. 2.VTT Technical Research Centre of Finland LtdEspooFinland

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