Establishment of an efficient micropropagation and callus regeneration system from the axillary buds of Bambusa ventricosa
- 876 Downloads
Based on the screening of various hormone combinations, we have established an efficient micropropagation and callus regeneration system from the axillary buds of B. ventricosa. We found that 6-benzyladenine (6-BA) had a dominant role in promoting bud sprouting, multiple bud induction and proliferation in B. ventricosa. Meanwhile α-naphthaleneacetic acid (NAA) was found to be an effective factor of inducing rooting in proliferated buds. The Murashige and Skoog medium containing 22.2 µM 6-BA was optimal for bud initiation, and the MS medium containing 26.6 µM 6-BA provided a good result for multiple bud induction. However, for buds proliferation MS medium containing 22.2 µM 6-BA, 0.23 µM Thidiazuron (TDZ: N-phenyl-N-[(1, 2, 3-thidiazol-5-yl) urea]) and 0.27 µM NAA was found to be very effective. The optimal medium for rooting of proliferated bud was MS medium containing 2.7 µM NAA, 4.9 µM indole butyric acid and 4.4 µM 6-BA. Based on the establishment of an efficient micropropagation system, we investigated the frequencies of callus formation from buds of in virto raised plantlets under different culture conditions. We showed that media containing 27 µM 2, 4-dichlorophenoxyacetic acid (2, 4-D), 2.7 µM NAA and 0.0045 µM TDZ effectively produced callus. The callus induction rate varied between it to 60 percent. TDZ was found to be a main factor influencing the callogenesis of B. ventricosa. Medium containing 22.6 µM 2, 4-D, 2.2 µM 6-BA and 5.4 µM NAA was preferred for callus amplification among the four tested subculture media. Plants were successfully regenerated on a MS medium containing 13.3 µM 6-BA and 2.7 µM NAA, subsequently acclimatized and transplanted to an experimental pod.
KeywordsBamboo Tissue culture Callogenesis Micropropagation Somatic variation
We thank Dr. Zhangjun Fei for critical reading of the manuscript. This work was supported by a Grant from the Natural Science Foundation of China (Grant No. 31301808), a grant from the Chinese Postdoctoral Science Foundation (Grant No. 2012M521090), a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, and a Grant from the National Science and Technology Support Program of China during the 12th Five-Year Plan Period (No. 2012BAD 23B05).
- Duncan DB (1955) Multiple range and multiple F tests. Biometrics 11:1–42Google Scholar
- Islam S, Rahman MM (2005) Micro-cloning in commercially important six bamboo species for mass propagation and at a large scale cultivation. Plant Tissue Cult Biotech 15:103–111Google Scholar
- Mehta U, Ramanuja Rao I, Mohan Ram H (1982) Somatic embryogenesis in bamboo. In: Akio Fujiwara (ed) Plant tissue culture 1982: proceedings, 5th international congress of plant tissue and cell culture held at Tokyo and Lake Yamanake, Japan, July 11–16Google Scholar
- Mudoi KD, Saikia SP, Goswami A, Gogoi A, Bora D, Borthakur M (2013) Micropropagation of important bamboos: a review. Afr J Biotechnol 12:2770–2785Google Scholar
- Peng ZH, Lu Y, Li LB, Zhao Q, Feng Q, Gao ZM, Lu HY, Hu T, Yao N, Liu KY, Li Y, Fan DL, Guo YL, Li WJ, Lu YQ, Weng QJ, Zhou CC, Zhang L, Huang T, Zhao Y, Zhu CR, Liu XG, Yang XW, Wang T, Miao K, Zhuang CY, Cao XL, Tang WL, Liu GS, Liu YL, Chen J, Liu ZJ, Yuan LC, Liu ZH, Huang XH, Lu TT, Fei BH, Ning ZM, Han B, Jiang ZH (2013b) The draft genome of the fast-growing non-timber forest species moso bamboo (Phyllostachys heterocycla). Nat Genet 45:456–461PubMedCrossRefGoogle Scholar
- Qiao G, Yang H, Zhang L, Han X, Liu M, Jiang J, Jiang Y, Zhuo R (2014) Enhanced cold stress tolerance of transgenic Dendrocalamus latiflorus Munro (Ma bamboo) plants expressing a bacterial CodA gene. Vitro Cell Dev Biol Plant 50(4):385–391Google Scholar
- Shih MC, Chou ML, Yue JJ, Hsu CT, Chang WJ, Ko SS, Liao DC, Huang YT, Chen JJW, Yuan JL, Gu XP, Lin CS (2014) BeMADS1 is a key to delivery MADSs into nucleus in reproductive tissues-De novo characterization of Bambusa edulis transcriptome and study of MADS genes in bamboo floral development. Bmc Plant Biol 14:179PubMedCentralPubMedCrossRefGoogle Scholar
- Singh M, Jaiswal U, Jaiswal V (2000) Thidiazuron-induced in vitro flowering in Dendrocalamus strictus Nees. Curr Sci 79:1529–1530Google Scholar