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Tissue culture and aromatic profile in Salvia dolomitica Codd

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Abstract

In this study, we developed tissue culture protocol of Salvia dolomitica, a particularly fragrant sage and we evaluated the yield and the profile of essential oil (EO) produced from plants grown in vivo compared to in vitro plant material cultured in two light intensity growth conditions. Plant extracts secreted from glandular trichomes are, in Lamiacea, a valuable source of biologically active volatile compounds. Microscopical observations permitted to describe, for the first time, secretory structures from S. dolomitica leaves developed in vitro showing morphological identity to in vivo glands. The yield of EO obtained from light-stressed in vitro plants was higher than which obtained from the non-stressed in vitro plants and threefold higher that in vivo plants. Phytochemical analysis of EOs and volatile compounds showed quali-quantitative differences between S. dolomitica in vitro grown plantlets and those from open field. α-Pinene, β-phellandrene and borneol accumulated predominantly in micropropagated plants while β-caryophyllene, α-guaiene, δ-cadinene and valencene in in vivo material. Both EO and SPME analysis had the same trend: the percentage of total sesquiterpenes decreased strongly in vitro, on the contrary the total monoterpenes are abundant. The effect of the high light treatment on aromatic profile demonstrating that this stress can affect the EO composition and this can be exploited by researchers to address the accumulation of valuable amounts of EO.

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References

  • Araújo C, Sousa MJ, Ferreira MF, Leão C (2003) Activity of essential oils from Mediterranean Lamiaceae species against food spoilage yeasts. J Food Prot 66:625–632

    PubMed  Google Scholar 

  • Arikat NA, Jawad FM, Karam NS, Shibli RA (2003) Micropropagation and accumulation of essential oils in wide sage (Salvia fruticosa Mill.). Sci Hortic 100:193–202

    Article  Google Scholar 

  • Avato P, Fortunato IM, Ruta C, D’Elia R (2005) Glandular hairs and essential oils in micropropagated plants of Salvia officinalis L. Plant Sci 169:29–36

    Article  CAS  Google Scholar 

  • Azam A, Qian J, Zhang B, Xu C, Chen K (2013) Citrus leaf volatiles as affected by developmental stage and genetic type. Int J Mol Sci 14(9):17744–17766

  • Burbott AJ, Loomis WD (1967) Effects of light and temperature on the monoterpenes of peppermint. Plant Physiol 42:20–28

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Caser M, Ruffoni B, Scariot V (2012) Screening for drought tolerance in Salvia spp. and Helichrysium petiolare: a way to select low maintenance ornamental plants. Act Hort 953:240–246

    Google Scholar 

  • Cervelli C, Capponi A, Mascarello B, Ruffoni B, Del Gaudio C (2013) New species and cultivars of Salvia as ornamental pot plants. Acta Hort 1000:35–41

    Google Scholar 

  • Chen JW, Cao KF (2005) Plant VOCs emission: a new strategy of thermotolerance. J For Res 16(4):323–326

    Article  CAS  Google Scholar 

  • Croteau R, Felton M, Karp F, Kjonaas R (1981) Relationship of camphor biosynthesis to leaf development in sage (Salvia officinalis). Plant Physiol 67:820–824

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Fahn A (1988) Secretory tissues in vascular plants. N Phytol 108(3):229–257

    Article  Google Scholar 

  • Fernandes VF, de Almeida LB, da S. Feijó EVR, da C. Silva D, de Oliveira RA, Mielke MS, do B Costa LC (2013) Light intensity on growth, leaf micromorphology and essential oil production of Ocimum gratissimum. Rev Bras Farmacogn 23(3):419–424

    Article  CAS  Google Scholar 

  • Fisher VL (2005) Indigenous Salvia species-an investigation of the antimicrobial activity, antioxidant activity and chemical composition of leaf extracts. MSc thesis

  • Grausgruber-Groger et al (2012) Seasonal influence on gene expression of monoterpene synthases in Salvia officinalis (Lamiaceae). J Plant Physiol 169:353–359

    Article  PubMed  Google Scholar 

  • Kamatou GPP, Viljoen AM, Gono-Bwalya AB, van Zyl RL, van Vuuren SF, Lourens ACU, Baser KHC, Demirci B (2005) The in vitro pharmacological activities and a chemical investigation of three South African Salvia species. J Ethnopharmacol 102:382–390

    Article  CAS  PubMed  Google Scholar 

  • Kamatou GPP, Viljoen AM, Figueiredo AC, Tilney PM, Van Zyl RL, Barroso JG, Pedro LG, Van Vuuren SF (2007a) Trichomes, essential oil composition and biological activities of Salvia albicaulis Benth. and S. dolomitica Codd, two species from the Cape region of South Africa. S Afr J Bot 73:102–108

    Article  CAS  Google Scholar 

  • Kamatou GPP, van Vuuren SF, van Heerden FR, Seaman T, Viljoen AM (2007b) Antibacterial ad antimycobacterial acitivities of South African Salvia species and isolated compounds from S. chamelaegnea. S Afr J Bot 73:552–557

    Article  CAS  Google Scholar 

  • Kamatou GPP, van Zyl RL, Davids H, van Heerden FR, Lourens ACU, Viljoen AM (2008) Antimalarial ad anticancer acitivities of selected South African Salvia species and isolated compounds from S. radula. S Afr J Bot 74:238–243

    Article  CAS  Google Scholar 

  • Kamatou GPP, Viljoen AM, Steenkamp P (2010) Antioxidant, anti-inflammatory activities and HPLC analysis of South African Salvia species. Food Chem 119:684–688

    Article  CAS  Google Scholar 

  • Marchev A, Haas C, Schulz S, Georgiev V, Steingroewer J, Bley T, Pavlov A (2014) Sage In vitro culture: a promising tool for the production of bioactive terpenes and phenolic substances. Biotechnol Lett 36:211–221

    Article  CAS  PubMed  Google Scholar 

  • Mascarello C, Mantovani E, Ruffoni B (2006) In vitro culture of several ornamental and medicinal Salvia species. Acta Hort 723:375–380

    Google Scholar 

  • Murashige T, Skoog F (1962) A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiol Plant 15(3):473–497

  • Ruffoni B, Raffi D, Rizzo A, Oleszek W, Giardi MT, Bertoli A, Pistelli L (2009) Establishment of in vitro Salvia cell biomass for the controlled production of antioxidant metabolites. Acta Hort 829:423–427

    CAS  Google Scholar 

  • Ruffoni B, Pistelli L, Bertoli A, Pistelli L (2010) Plant cell cultures: bioreactors for industrial production. Adv Exp Med Biol 698:203–218

    Article  CAS  PubMed  Google Scholar 

  • Santos-Gomes PC, Fernandes-Ferreira M (2001) Organ- and season-dependent variation in the essential oil composition of Salvia officinalis L. cultivated at two different sites. J Agric Food Chem 49(6):2908–2916

    Article  CAS  PubMed  Google Scholar 

  • Schnitzler WH, Habegger R (2004) Perilla frutescens-Perilla red and its secondary plant metabolism. Acta Hort 659:371–374

    CAS  Google Scholar 

  • Shoemaker M, Hamilton B, Dairkee SH, Cohen I, Campbell MJ (2005) In vitro anticancer activity of twelve Chinese medicinal herbs. Phytother Res 19(7):649–651

    Article  PubMed  Google Scholar 

  • Wang JW, Wu JY (2010) Tanshinone biosynthesis in Salvia miltiorrhiza and production in plant tissue cultures. Appl Microbiol Biotechnol 88:437–449

    Article  CAS  PubMed  Google Scholar 

  • Watt JM, Breyer-Brandwijk MG (1962) The medicinal and poisonous plants of southern and eastern Africa. Livingstone, Edinburgh

    Google Scholar 

  • Zuzarte MR, Dinis AM, Cavaleiro C, Salgueiro LR, Canhoto JM (2010) Trichomes, essential oils and in vitro propagation of Lavandula peduncolata (Lamiaceae). Ind Crops Prod 32:580–587

    Article  CAS  Google Scholar 

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Acknowledgments

L.B. was supported by the BIOAROMA project financed by Regione Liguria POR FSO, E.G. was supported by Fondazione CARIGE. The authors express their gratitude to Pietro Guglielmi for technical assistance in EO isolation and Pasquale Casella and Sergio Ariano for plant care and management.

Conflict of interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Consiglio per la Ricerca e la Sperimentazione in Agricoltura - CRA-FSO Ornamental Plant Research Unit, Sanremo, IM, Italy

    L. Bassolino, A. Cassetti & B. Ruffoni

  2. Dipartimento di Medicina Sperimentale e Centro di Eccellenza per la Ricerca Biomedica (DIMES), Università di Genova, Viale Benedetto XV 7, 16132, Genoa, Italy

    E. Giacomelli & G. Damonte

  3. Dipartimento di Farmacia, Università di Pisa, Via Bonanno, 6, 56126, Pisa, Italy

    S. Giovanelli & L. Pistelli

  4. Dipartimento di Farmacia (DIFAR), Università di Genova, Via Brigata Salerno, 13, 16132, Genoa, Italy

    A. Bisio

Authors
  1. L. Bassolino
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  2. E. Giacomelli
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  3. S. Giovanelli
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  4. L. Pistelli
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  5. A. Cassetti
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  6. G. Damonte
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  7. A. Bisio
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  8. B. Ruffoni
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Corresponding author

Correspondence to B. Ruffoni.

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Bassolino, L., Giacomelli, E., Giovanelli, S. et al. Tissue culture and aromatic profile in Salvia dolomitica Codd. Plant Cell Tiss Organ Cult 121, 83–95 (2015). https://doi.org/10.1007/s11240-014-0681-3

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  • DOI: https://doi.org/10.1007/s11240-014-0681-3

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