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Current status of tissue culture and genetic transformation research in cotton (Gossypium spp.)

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An Erratum to this article was published on 29 January 2015

Abstract

Cotton (Gossypium spp.) is an economically very important fiber yielding crop, which is grown almost in sixty-five countries throughout the world. Like other crops, cotton also suffers from major biotic and abiotic stresses. In fact, the losses due to insect pests in cotton are enormous compared to other crops, thereby reducing the actual economic potential. It is a well-known fact that more than half of the total pesticide consumption across the world is utilized on controlling insect pests in this crop. Though conventional breeding and integrated pest management practices have resulted in improving/developing fiber quality, heat tolerance, CMS lines and yield, much success has not been reported with respect to biotic and abiotic stresses, especially insect pests due to the non-availability of genes conferring resistance within a crossable gene pool. Thus, genetic engineering has become an inevitable tool in finding solutions to these problems and transfer of alien genes into commercially important cotton varieties in the last two decades. In fact ~81 % of cotton grown throughout the world is genetically modified. Despite these achievements, several limitations still exist in achieving cotton transformation. In this review, we discuss the status of different regeneration and transformation methods in cotton along with the major factors that exert influence in developing cotton transgenics, besides the chronological progress made in tissue culture and cotton transformation technology.

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Abbreviations

2iP:

N 6-[2-Isopentyl] adenine

2,4-D:

2,4-Dichlorophenoxyacetic acid

AgNO3 :

Silver nitrate

BAP:

Benzyl amino purine

cry1Ab :

Crystalline protein 1Ab

cry1Ac :

Crystalline protein 1Ac

DPA:

Days post anthesis

GA3 :

Gibberellic acid 3

KIN:

Kinetin

NAA:

α-Naphthalene acetic acid

TDZ:

Thiadiazuron

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Acknowledgments

Author V.N.J. is greatly thankful to Agri Biotech Foundation (Formerly AP Netherlands Biotechnology Programme) Hyderabad, India for awarding fellowship. The authors also thank G. Pakki Reddy (Executive Director, Agri Biotech Foundation, Hyderabad, India), J. S. Bentur and G. Mallikarjuna of the Agri Biotech Foundation for their suggestions in preparing the manuscript.

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  1. Plant Molecular Biology Laboratory, Agri Biotech Foundation (Formerly AP Netherlands Biotechnology Programme), Hyderabad, 500030, India

    Vijaya Naresh Juturu & Gopala Krishna Mekala

  2. Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, 500046, India

    P. B. Kirti

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  1. Vijaya Naresh Juturu
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  2. Gopala Krishna Mekala
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Juturu, V.N., Mekala, G.K. & Kirti, P.B. Current status of tissue culture and genetic transformation research in cotton (Gossypium spp.). Plant Cell Tiss Organ Cult 120, 813–839 (2015). https://doi.org/10.1007/s11240-014-0640-z

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