Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 120, Issue 3, pp 813–839 | Cite as

Current status of tissue culture and genetic transformation research in cotton (Gossypium spp.)

  • Vijaya Naresh Juturu
  • Gopala Krishna Mekala
  • P. B. Kirti
Review

Abstract

Cotton (Gossypium spp.) is an economically very important fiber yielding crop, which is grown almost in sixty-five countries throughout the world. Like other crops, cotton also suffers from major biotic and abiotic stresses. In fact, the losses due to insect pests in cotton are enormous compared to other crops, thereby reducing the actual economic potential. It is a well-known fact that more than half of the total pesticide consumption across the world is utilized on controlling insect pests in this crop. Though conventional breeding and integrated pest management practices have resulted in improving/developing fiber quality, heat tolerance, CMS lines and yield, much success has not been reported with respect to biotic and abiotic stresses, especially insect pests due to the non-availability of genes conferring resistance within a crossable gene pool. Thus, genetic engineering has become an inevitable tool in finding solutions to these problems and transfer of alien genes into commercially important cotton varieties in the last two decades. In fact ~81 % of cotton grown throughout the world is genetically modified. Despite these achievements, several limitations still exist in achieving cotton transformation. In this review, we discuss the status of different regeneration and transformation methods in cotton along with the major factors that exert influence in developing cotton transgenics, besides the chronological progress made in tissue culture and cotton transformation technology.

Keywords

Genetic transformation Gossypium spp. In planta transformation Organogenesis Somatic embryogenesis 

Abbreviations

2iP

N6-[2-Isopentyl] adenine

2,4-D

2,4-Dichlorophenoxyacetic acid

AgNO3

Silver nitrate

BAP

Benzyl amino purine

cry1Ab

Crystalline protein 1Ab

cry1Ac

Crystalline protein 1Ac

DPA

Days post anthesis

GA3

Gibberellic acid 3

KIN

Kinetin

NAA

α-Naphthalene acetic acid

TDZ

Thiadiazuron

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Vijaya Naresh Juturu
    • 1
  • Gopala Krishna Mekala
    • 1
  • P. B. Kirti
    • 2
  1. 1.Plant Molecular Biology LaboratoryAgri Biotech Foundation (Formerly AP Netherlands Biotechnology Programme)HyderabadIndia
  2. 2.Department of Plant Sciences, School of Life SciencesUniversity of HyderabadHyderabadIndia

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