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Agrobacterium tumefaciens-mediated transformation of blackberry (Rubus fruticosus L.)

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Abstract

The aim of work was to elaborate the system for Agrobacterium tumefaciens-mediated transformation and transgenic plant regeneration in Rubus fruticosus L. using explants from mature plants. Regeneration of transgenic shoots was achieved from cut ends of petioles completely immersed in the medium using flag explants cultivated vertically on MS medium with 1 mg l−1 TDZ and 0.02 mg l−1 IBA followed by transfer on medium with 1 mg l−1 BAP, 0.02 mg l−1 IBA and 0.1 mg l−1 GA3, both supplemented with 10 and 15 mg l−1 hygromycin after transformation by A. tumefaciens strain LBA 4404/pCambia 1304. Four putative transgenic plants of cv. ‘Čačanska Bestrna’ were rooted, acclimatized and analysed. Transgenic character of the tissue from analysed plants was confirmed by nested PCR and subsequent sequencing.

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Acknowledgments

The work was supported by VEGA, project no. 2/0040/11 and 2/0140/14, SRDA project Slovakia-Serbia no. SK-SRB-0033-11 and European Community project no. 26220220180: Building Research Centre “AgroBioTech”.

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Authors and Affiliations

  1. Institute of Plant Genetics and Biotechnology, SAS, 950 07, Nitra, Slovakia

    Miroslava Súkeníková, Gabriela Libiaková, Jana Moravčíková, Andrea Hricová & Alena Gajdošová

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  1. Miroslava Súkeníková
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  2. Gabriela Libiaková
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  3. Jana Moravčíková
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  4. Andrea Hricová
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  5. Alena Gajdošová
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Correspondence to Alena Gajdošová.

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Súkeníková, M., Libiaková, G., Moravčíková, J. et al. Agrobacterium tumefaciens-mediated transformation of blackberry (Rubus fruticosus L.). Plant Cell Tiss Organ Cult 120, 351–354 (2015). https://doi.org/10.1007/s11240-014-0569-2

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  • DOI: https://doi.org/10.1007/s11240-014-0569-2

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