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Cryopreservation of ex-vitro-grown Rosa chinensis ‘Old Blush’ buds using droplet-vitrification and encapsulation-dehydration

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Abstract

Axillary buds from greenhouse-grown plants of Rosa chinensis ‘Old Blush’ were successfully used to establish cryopreservation protocols using both droplet-vitrification and encapsulation-dehydration methods. In droplet vitrification, regrowth occurred after exposure to liquid nitrogen even without pre-culture in the loading solution (LS) before immersion in the plant vitrification solution 2 (PVS2). However, a 20–80 min LS step followed by a short immersion in PVS2 for 3 or 15 min, at 0 °C gave the best regrowth rates (82–86 %). In encapsulation dehydration, the level of dehydration significantly influenced shoot regrowth. The best regrowth rate, 60 %, was obtained at a bead water content of 0.35 g water per g dry weight. These results demonstrate the possibility of using greenhouse plants of rose for cryopreservation by droplet vitrification and encapsulation dehydration.

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Acknowledgments

We would like to thank Tatiana Thouroude for the transfer of the first ‘Old Blush’ cuttings, Linda Voisine for her participation in the preliminary tests on medium culture, Daniel Relion for his technical help in maintaining the greenhouse-grown rosebushes and the INEM team for greenhouse supervision. The authors are also very grateful to D. Goodfellow for reviewing the English manuscript.

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Authors and Affiliations

  1. AGROCAMPUS OUEST, UMR1345 Institut de Recherche en Horticulture et Semences, 2, rue Le Nôtre, 49045, Angers, France

    Camille Le Bras, Pierre-Henri Le Besnerais, Latifa Hamama & Agnès Grapin

  2. INRA, UMR1345 Institut de Recherche en Horticulture et Semences, 49071, Angers, France

    Camille Le Bras, Latifa Hamama & Agnès Grapin

  3. Université d’Angers, UMR1345 Institut de Recherche en Horticulture et Semences, 49071, Angers, France

    Camille Le Bras, Latifa Hamama & Agnès Grapin

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  1. Camille Le Bras
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  2. Pierre-Henri Le Besnerais
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  3. Latifa Hamama
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  4. Agnès Grapin
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Correspondence to Agnès Grapin.

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Le Bras, C., Le Besnerais, PH., Hamama, L. et al. Cryopreservation of ex-vitro-grown Rosa chinensis ‘Old Blush’ buds using droplet-vitrification and encapsulation-dehydration. Plant Cell Tiss Organ Cult 116, 235–242 (2014). https://doi.org/10.1007/s11240-013-0400-5

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  • DOI: https://doi.org/10.1007/s11240-013-0400-5

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