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Potato leafroll virus (PLRV) and Potato virus Y (PVY) influence vegetative growth, physiological metabolism, and microtuber production of in vitro-grown shoots of potato (Solanum tuberosum L.)

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Abstract

Effects of Potato leafroll virus (PLRV) and Potato virus Y (PVY) on vegetative growth, physiological metabolism and microtuber production were investigated using in vitro shoot cultures. The results showed that parameters of shoot growth including bud break percentage, shoot length, and node number and length were markedly reduced in the diseased shoots. These negative effects were much more pronounced in shoots co-infected with PLRV and PVY than in those singly infected with either PLRV or PVY. The inhibitive effects on root developments measured by root number and length were observed only in shoots co-infected with PLRV and PVY. Significantly lower contents of chl-a, chl-b and total chl were found in virus infected shoots than in healthy ones. There were striking differences in contents of total soluble protein observed between healthy shoots and PLVR and PVY co-infected ones. The content of total soluble sugar was highest in shoots co-infected with PLRV and PVY, and lowest in healthy shoots. Furthermore, there were no significant differences found in the level of endogenous indole-acetic acid among healthy shoots verses virus infected shoots. However, the level of zeatin-ribosome was much higher in healthy shoots than in virus infected ones. Yet, both healthy and single PLRV infected shoots produced similar levels of gibberillic acid 3, which were much higher than those of PVY single-infected shoots and PLRV and PVY co-infected shoots. Also, there were no significant differences in the number of microtubers among healthy shoots, PLVR single or PVY single infected shoots, but shoots co-infected with PLRV and PVY produced the lowest number of microtubers. Overall, the healthy shoots produced the largest size of microtubers and the highest percentage of microtubers ≥5 mm in diameter.

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Abbreviations

BM:

Basal medium

Chl-a:

Chlorophyll a

Chl-b:

Chlorophyll b

D:

Density

DAS-ELISA:

Sandwich enzyme-linked immunological assay

FV:

Final volume

FW:

Fresh weight

GA3 :

Gibberillic acid 3

IAA:

Indole-acetic acid

JA:

Jasmonic acid

KT:

Kinetin

MS:

Murashige and Skoog medium

MTPM:

Microtuber production medium

PLRV:

Potato leafroll virus

PVA:

Potato virus A

PVM:

Potato virus M

PVPP:

Polyvinylpolypyrolidone

PVS:

Potato virus S

PVX:

Potato virus X

PVY:

Potato virus Y

RT-PCR:

Reverse-transcript polymerase chain reaction

ScYLV:

Sugarcane yellow leaf virus

T chl:

Total chlorophyll

TFW:

Total fresh weight

ZR:

Zeatin-ribosome

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Acknowledgments

The authors would like to acknowledge financial supports from Department of Science and Technology of Shaanxi Province through a key project “13115” (2009ZDKG-10), Department of Science and technology of Yulin City (2011KJZX04) and Northwest A&F University through a president foundation.

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Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology in Arid Areas, Key Laboratory of Genetic Improvement of Horticultural Crops of Northwest China, Ministry of Agriculture of China, College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Jing-Wei Li, Biao Wang, Xiao-Ming Song, Ren-Rui Wang, Long Chen, Hao Zhang, Zhi-Bo Zhang & Qiao-Chun Wang

  2. Institute of Animal and Plant Quarantine, Chinese Academy of Inspection and Quarantine (CAIQ), Chaoyang District, Beijing, 10029, People’s Republic of China

    Ren-Rui Wang

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  1. Jing-Wei Li
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  2. Biao Wang
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Correspondence to Qiao-Chun Wang.

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Jing-Wei Li and Biao Wang contributed equally to the present study.

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Li, JW., Wang, B., Song, XM. et al. Potato leafroll virus (PLRV) and Potato virus Y (PVY) influence vegetative growth, physiological metabolism, and microtuber production of in vitro-grown shoots of potato (Solanum tuberosum L.). Plant Cell Tiss Organ Cult 114, 313–324 (2013). https://doi.org/10.1007/s11240-013-0327-x

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  • DOI: https://doi.org/10.1007/s11240-013-0327-x

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