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Stress-induced expression and structure of the putative gene hyp-1 for hypericin biosynthesis

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Abstract

The phenolic oxidative coupling protein (Hyp-1) with proposed activity in the biosynthesis of hypericin in Hypericum perforatum shares about 50 % sequence similarity with Bet.v.1-like/PR-10 proteins. In our previous study, we showed that this protein is not a limiting factor in hypericin biosynthesis. To ascertain the role of Hyp-1 in defense mechanisms, we have analyzed some structural features of the hyp-1 gene in 14 Hypericum species with different abilities to synthesise hypericin. We show that the hyp-1 gene possesses characteristics typical for genes encoding plant PR-10 proteins. The coding sequence of the hyp-1 gene is interrupted by a single 86- to 125-bp intron localised strictly in codon 62, which is a typical feature of the dicot PR-10 subfamily. The localisation of the intron is conserved in all 14 tested Hypericum species indicating a common evolutionary history with genes encoding PR-10 proteins. In addition, we report that the hyp-1 gene exhibits a similar response to stress conditions as the PR-10 proteins encoding genes. Following either wounding or infection by Agrobacterium tumefaciens, all analysed Hypericum species exhibited rapid and significant upregulation of hyp-1 gene expression; this was particularly observed in hypericin-producing species. On the other hand, in the presence of high levels of abscisic acid, different levels of gene expression were observed.

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Abbreviations

ABA:

Abscisic acid

Hyp-1:

Phenolic-coupling protein

PR-10:

Pathogenesis related class-10 proteins

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Acknowledgments

This work was supported by the Slovak Research and Development Agency under contracts No 0040-10 and LPP-0021-09 and by the Scientific Grant Agency of the Slovak Republic 1/142/11 and by Structural Funds of EU under contract No 007/20092.1/OPVaV.

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Authors and Affiliations

  1. Faculty of Science, Institute of Biology and Ecology, P. J. Šafárik University, Mánesova 23, 041 54, Košice, Slovakia

    Ján Košuth, Denisa Hrehorová & Eva Čellárová

  2. Department of Crystallography, Faculty of Chemistry, A. Mickiewicz University, Grunwaldzka 6, 60-780, Poznan, Poland

    Mariusz Jaskolski

  3. Center for Biocrystallographic Research, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704, Poznan, Poland

    Mariusz Jaskolski

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  1. Ján Košuth
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  2. Denisa Hrehorová
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  3. Mariusz Jaskolski
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  4. Eva Čellárová
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Correspondence to Eva Čellárová.

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Košuth, J., Hrehorová, D., Jaskolski, M. et al. Stress-induced expression and structure of the putative gene hyp-1 for hypericin biosynthesis. Plant Cell Tiss Organ Cult 114, 207–216 (2013). https://doi.org/10.1007/s11240-013-0316-0

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