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Identification of an AtCRN1-like chloroplast protein BeCRN1 and its distinctive role in chlorophyll breakdown during leaf senescence in bamboo (Bambusa emeiensisViridiflavus’)

  • Qiang Wei
  • Huiming Cao
  • Zhongru Li
  • Benke Kuai
  • Yulong DingEmail author
Original Paper

Abstract

CRN1/PPH/NYC3 is one of the key genes responsible for chlorophyll degradation during senescence in both Arabidopsis and rice. In this study, BeCRN1 and its promoter, BeCRN1p, were isolated from, Bambusa emeiensis ‘Viridiflavus’, a bamboo variety, for the first time. BeCRN1 consists of 1,646 bp, with an open reading frame of 1,473 bp, encoding a predicted polypeptide of 490 amino acids. Besides, BeCRN1 contained 5 exons and 4 introns, and harbored a distinctive microsatellite in the fourth intron. Differences in BeCRN1p are observed primarily in the AtCRN1 promoter, while sharing similar cis element composition with the rice CRN1 promoter. BeCRN1 is localized within the chloroplast, and it is strongly induced by senescence signals. Although constitutive overexpression of BeCRN1 in crn1 has reversed the stay-green phenotype of crn1 to the wild type phenotype, its promoter has failed to do so with AtCRN1 following dark treatment. The efficiency of BeCRN1p is only about one-third of that of the AtCRN1 promoter.

Keywords

Bamboo Chlorophyll degradation CRN1 Stay green 

Notes

Acknowledgments

This work was supported by a grant from the Natural Science Foundation of China (Grant No. 31000294), a grant from the National Science and Technology Support Program of China during the 12th Five-Year Plan Period (No. 2012BAD23B05), and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Qiang Wei
    • 1
  • Huiming Cao
    • 1
  • Zhongru Li
    • 1
  • Benke Kuai
    • 2
  • Yulong Ding
    • 1
    Email author
  1. 1.Bamboo Research Institute and College of Forest Resources and EnvironmentNanjing Forestry UniversityNanjingChina
  2. 2.State Key Laboratory of Genetic Engineering and Institute of Plant Biology, School of Life SciencesFudan UniversityShanghaiChina

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