Recently, the use of plants for the production of recombinant proteins has been well demonstrated with promising outcomes. In this study, an efficient Nicotiana tabacum L. cv. Bright Yellow 2 (BY-2) cells system expressing the ectodomain of hemagglutinin-neuraminidase (eHN) protein from Newcastle disease virus (NDV) strain AF2240 was established. Transgenic tobacco BY-2 cell cultures expressing the immunogenic eHN protein were generated and the translation efficiency of eHN protein was enhanced using the 5′-untranslated region of Nicotiana tabacum alcohol dehydrogenase gene (NtADH 5′-UTR) under the control of strong cauliflower mosaic virus (CaMV 35S) promoter. Transgenic lines verified by real-time PCR showed high level of eHN mRNA transcripts and immunoblotting confirmed the presence of 66 kD eHN protein. The eHN protein was stably produced in an average of 0.2–0.4 % total soluble protein. Green fluorescent protein-tagged eHN protein was expressed and localized at the cytosol of BY-2 cell. All mice receiving purified eHN protein from transgenic tobacco BY-2 cells produced specific anti-NDV antibodies. We concluded that plant made eHN elicit immune response and can serve as candidate vaccine against NDV.
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Ectodomain of hemagglutinin-neuraminidase
Green fluorescent protein
Linsmaier and Skoog
- NtADH 5′-UTR:
5′-Untranslated region of Nicotiana tabacum alcohol dehydrogenase gene
Newcastle disease virus
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Authors are thankful to the members of plant biotechnology and virology laboratories in UPM for their technical support.
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Lai, K.S., Yusoff, K. & Mahmood, M. Functional ectodomain of the hemagglutinin-neuraminidase protein is expressed in transgenic tobacco cells as a candidate vaccine against Newcastle disease virus. Plant Cell Tiss Organ Cult 112, 117–121 (2013). https://doi.org/10.1007/s11240-012-0214-x
- Newcastle disease virus
- NtADH 5′-UTR
- Plant-based vaccine