Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 112, Issue 1, pp 19–31 | Cite as

Overexpression of a poplar two-pore K+ channel enhances salinity tolerance in tobacco cells

  • Feifei Wang
  • Shurong Deng
  • Mingquan Ding
  • Jian Sun
  • Meijuan Wang
  • Huipeng Zhu
  • Yansha Han
  • Zedan Shen
  • Xiaoshu Jing
  • Fan Zhang
  • Yue Hu
  • Xin Shen
  • Shaoliang Chen
Original Paper


Populus euphratica is a plant model intensively studied for elucidating physiological and molecular mechanisms of salt tolerance in woody species. Several studies have shown that vacuolar potassium (K+) ion channels of the two-pore K+ (TPK) family play an important role in maintaining K+ homeostasis. Here, we cloned a putative TPK channel gene from P. euphratica, termed PeTPK. Sequence analysis of PeTPK1 identified the universal K-channel-specific pore signature, TXGYGD. Over-expression of PeTPK1 in tobacco BY-2 cells improved salt tolerance, but did not enhance tolerance to hyperosmotic stress caused by mannitol (200–600 mM). After 3 weeks of NaCl stress (100 and 150 mM), PeTPK1-transgenic cells had higher fresh and dry weights than wild-type cells. Salt treatment caused significantly higher Na+ accumulation and K+ loss in wild-type cells compared to transgenic cells. During short-term salt stress (100 mM NaCl, 24-h), PeTPK1-transgenic cells showed higher cell viability and reduced membrane permeabilization compared to wild-type cells. Scanning ion-selective electrode data revealed that salt-shock elicited a significantly higher transient K+ efflux from PeTPK1-transgenic callus cells and protoplasts compared to that observed in wild-type cells and protoplasts. We concluded that salt tolerance in P. euphratica is most likely mediated through PeTPK1. We propose that, under salt stress, PeTPK1 functions as an outward-rectifying, K+ efflux channel in the vacuole that transfers K+ to the cytosol to maintain K+ homeostasis.


K+ flux NaCl PeTPK1 Populus euphratica Protoplast Tobacco BY-2 cells 



The research was supported jointly by the Fundamental Research Funds for the Central Universities (JC2011-2), the National Natural Science Foundation of China (31170570, 30872005), the Foundation for the Supervisors of Beijing Excellent Doctoral Dissertations (YB20081002201), the Beijing Natural Science Foundation (6112017), and the Key Projects of the Ministry of Education, PR China (209084). We thank Ms. Junqi Zhang and Meiqin Liu for their assistance in confocal analysis.


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Feifei Wang
    • 1
  • Shurong Deng
    • 1
  • Mingquan Ding
    • 1
    • 2
  • Jian Sun
    • 1
    • 3
  • Meijuan Wang
    • 1
  • Huipeng Zhu
    • 1
  • Yansha Han
    • 1
  • Zedan Shen
    • 1
  • Xiaoshu Jing
    • 1
  • Fan Zhang
    • 1
  • Yue Hu
    • 1
  • Xin Shen
    • 1
  • Shaoliang Chen
    • 1
  1. 1.College of Biological Sciences and Technology (Box 162)Beijing Forestry UniversityBeijingPeople’s Republic of China
  2. 2.College of Agricultural and Food ScienceZhejiang Agricultural and Forestry UniversityHangzhouPeople’s Republic of China
  3. 3.College of Life ScienceJiangsu Normal UniversityXuzhouPeople’s Republic of China

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