Skip to main content
SpringerLink
Log in

Callus induction and shoot organogenesis from anther cultures of Curcuma attenuata Wall

  • Original Paper
  • Published:
Plant Cell, Tissue and Organ Culture (PCTOC) Aims and scope Submit manuscript

Abstract

Curcuma attenuata is a highly valued ornamental. This study provides the first report on C. attenuata shoot organogenesis and plant regeneration. Immature anthers derived from 5 to 7 cm long inflorescences were isolated and cultured on different variations of Murashige and Skoog (MS) media to induce callus and then shoot organogenesis. When the 2-mm long anthers in which microspores were at the uninucleate developmental stage were cultured in the dark on MS medium containing 13.6 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 2.3 μM kinetin (KT) for 15 days and then transferred to 40 μmol m−2 s−1 fluorescent light for 30 days, the percentage callus induction reached 33.3 %. After callus was transferred to various differentiation media and cultured in the light, 33.1 % of all callus cultures could differentiate into adventitious shoots on MS medium supplemented with 22.0 μM 6-benzyladenine (BA), 0.53 μM α-naphthaleneacetic acid (NAA) and 1.4 μM thidiazuron (TDZ) after culturing for 60 days. Over 95 % of plantlets survived after transplanting plantlets into trays with a mixture of sand and perlite (2: 1) for 20 days. Chromosome number, determined from the root tips of young plantlets, indicated that all plantlets were diploid (2n = 84).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

Abbreviations

BA:

6-Benzyladenine

2,4-D:

2,4-Dichlorophenoxyacetic acid

NAA:

α-Naphthaleneacetic acid

TDZ:

Thidiazuron

KT:

Kinetin

PGR:

Plant growth regulator

MS:

Murashige and Skoog

References

  • Apavatjrut P, Sirisawad T, Sirirugsa P, Voraurai P, Suwanthada C (1996) Studies on chromosome number of seventeen Thai Curcuma species. In: Proceedings of 2nd national conference on flower and ornamental plant, vol 2, pp 86–99

  • Balachandran SM, Bhat SR, Chandel KPS (1990) In vitro clonal multiplication of turmeric (Curcuma spp.) and ginger (Zingiber officinale Rosc.). Plant Cell Rep 8:521–552

    Article  CAS  Google Scholar 

  • Jana LS, Otakar S, Vlasta J, Mamyil S, Tomáš F, Pavel T, Suda J (2007) Chromosome numbers and genome size variation in Indian species of Curcuma (Zingiberaceae). Ann Bot 100:505–526

    Article  Google Scholar 

  • Mohanty S, Panda MK, Subudhi E, Nayak S (2008) Plant regeneration from callus culture of Curcuma aromatica and in vitro detection of somaclonal variation through cytophotometric analysis. Biol Plant 52:783–786

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nayak S (2000) In vitro multiplication and microrhizome induction in Curcuma aromatica Salisb. Plant Growth Regul 32:41–47

    Article  CAS  Google Scholar 

  • Prakash S, Elangomathavan RE, Seshadri S, Kathiravan K, Ignacimuthu S (2004) Efficient regeneration of Curcuma amada Roxb. plantlets from rhizome and leaf sheath explants. Plant Cell Tissue Org Cult 78:159–165

    Article  CAS  Google Scholar 

  • Prathanturarug S, Soonthornchareonnon N, Chuakul W, Phaidee Y, Saralamp P (2003a) High-frequency shoot multiplication in Curcuma longa L. using thidiazuron. Plant Cell Rep 21:1054–1059

    Article  PubMed  CAS  Google Scholar 

  • Prathanturarug S, Soonthornchareonnon N, Chuakul W, Phaidee Y, Saralamp P (2003b) High-frequency shoot multiplication in Curcuma longa L. using thidiazuron. Plant Cell Rep 21:1054–1059

    Article  PubMed  CAS  Google Scholar 

  • Prathanturarug S, Soonthornchareonnon N, Chuakul W, Phaidee Y, Saralamp P (2005) Rapid micropropagation of Curcuma longa using bud explants pre-cultured in thidiazuron supplemented liquid medium. Plant Cell Tissue Org Cult 80:347–351

    Article  CAS  Google Scholar 

  • Purseglove JW, Brown EG, Green CL, Robbins SR (1981) Spices. Longman, London, New York

    Google Scholar 

  • Salvi ND, Geoge L, Eapen S (2000) Direct regeneration of shoots from immature inflorescence cultures of turmeric. Plant Cell Tissue Org Cult 62:235–238

    Article  CAS  Google Scholar 

  • Salvi ND, George L, Eapen S (2001) Plant regeneration from leaf base callus of turmeric and random amplified polymorphic DNA analysis of regenerated plants. Plant Cell Tissue Org Cult 66:113–119

    Article  CAS  Google Scholar 

  • Samsudeen K, Babu KN, Divakaran M, Ravindran PN (2000) Plant regeneration from anther derived callus cultures of ginger (Zingiber officinale Rosc.). J Hortic Sci Biotech 75:447–450

    CAS  Google Scholar 

  • Schaffer M, Schaffer PM, Zidan J, Sela GB (2011) Curcuma as a functional food in the control of cancer and inflammation. Curr Opin Clinical Nutrition Metabolic Care 4:588–597

    Article  Google Scholar 

  • Sit AK, Tiwari RS (1997) Micropropagation of turmeric (Curcuma longa L.). Rec Hortic 4:145–148

    Google Scholar 

  • Sunitibala H, Damayanti M, Sharma GJ (2001) In vitro propagation and rhizome formation in Curcuma longa Linn. Cytobios 105:71–82

    PubMed  CAS  Google Scholar 

  • Toppoonyanont N, Chongsang S, Chujan S, Somsueb S, Nuamjaroen P (2005) Micropropagation scheme of Curcuma alismatifolia Gagnep. Acta Hort (ISHS) 673:705–712

    Google Scholar 

  • Tyagi RK, Yusuf A, Dua P, Agrawal A (2004) In vitro plant regeneration and genotype conservation of eight wild species of Curcuma. Biol Plant 48:129–132

    Article  CAS  Google Scholar 

  • Udomdee W, Fukai S, Petpradap L, Teixeira da Silva JA (2003) Curcuma: studies on tissue culture, pollen germination and viability, histology and flow cytometry. Prop Ornamental Plants 3:34–41

    Google Scholar 

  • Viu AFM, Viu MAO, Tavares AR, Vianello F, Lima GPP (2009) Endogenous and exogenous polyamines in the organogenesis in Curcuma longa L. Sci Hortic 121:501–504

    Article  CAS  Google Scholar 

  • Wallich N (1828) A numerical list of dried specimens of plants in the East India company’s Museum: collected under the superintendence of Dr. Wallich of the company’s botanic garden at Calcutta. Treuttel and Wurtz, London, pp 6225–7683

    Google Scholar 

  • Wannakrairoj S (1997) Clonal micropropagation of patumma (Curcuma alismatifolia gagnep). Witthayasan Kasetsart 31:353–356

    Google Scholar 

  • Xu Q, Teixeira da Silva JA, Kong L (2006) Advanced biotechnology used in Curcuma plant research. In: Teixeira da Silva JA (ed) Floriculture, ornamental and plant biotechnology: advances and topical issues, 1st edn, vol IV. Global Science Books, Ltd., Isleworth, UK, pp 517–528

  • Zhang XH, Teixeira da Silva JA, Ma GH (2010) Karyotype analysis of Santalum album L. Caryologia 63:142–148

    Google Scholar 

  • Zhang SJ, Liu N, Sheng AW, Ma GH, Wu GJ (2011) In vitro plant regeneration from organogenic callus of Curcuma kwangsiensis Lindl. (Zingiberaceae). Plant Growth Regul 64:141–145

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was financially supported by the Science and Technology Planning Project of Guangdong Province (2010B020305013), which is gratefully acknowledged.

Author information

Authors and Affiliations

  1. College of Agriculture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, China

    Yaping Kou & Nian Liu

  2. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, The Chinese Academy of Sciences, Guangzhou, 510650, People’s Republic of China

    Guohua Ma

  3. Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, Miki-cho, Ikenobe 2393, Kagawa-ken, 761-0795, Japan

    Jaime A. Teixeira da Silva

Authors
  1. Yaping Kou
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Guohua Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jaime A. Teixeira da Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Nian Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Guohua Ma, Jaime A. Teixeira da Silva or Nian Liu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kou, Y., Ma, G., Teixeira da Silva, J.A. et al. Callus induction and shoot organogenesis from anther cultures of Curcuma attenuata Wall. Plant Cell Tiss Organ Cult 112, 1–7 (2013). https://doi.org/10.1007/s11240-012-0205-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-012-0205-y

Keywords

Search

Navigation