In vitro conservation of Bacopa monnieri (L.) using mineral oil


Bacopa monnieri (L.) Wettst., a traditional Indian medicinal plant with high commercial potential, is used as a potent nervine tonic. A slow growth protocol was developed for medium-term conservation using mineral oil (MO) overlay. Nodal segments of B. monnieri (two genotypes; IC249250, IC468878) were conserved using MO for 24 months. Single node explants were implanted on MS medium supplemented with 0.2 mg l−1 BA and were covered with MO. Subculture duration could be significantly enhanced from 6 to 24 months, on the above medium. Normal plants regenerated from conserved cultures were successfully established in soil. On the basis of 20 random amplified polymorphic DNA and 5 inter-simple sequence repeat primers analyses and bacoside A content using HPLC, no significant reproducible variation was observed between the controls and in vitro-conserved plants. The results demonstrate the feasibility of using MO for medium-term conservation of B. monnieri germplasm without any adverse genetical and biochemical effects.

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Mineral oil


Murashige and Skoog’s medium


Nodal segment


Unweighted pair group method with arithmetic mean


Random amplified polymorphic DNA


Inter-simple sequence repeat


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We thank Director, National Bureau of Plant Genetic Resources, New Delhi for facilities and encouragement. Thanks are due to Sh. Ramesh Chandra for technical assistance. Financial support provided by the Department of Biotechnology, Ministry of Science and Technology, is gratefully acknowledged.

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Correspondence to Neelam Sharma.

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Sharma, N., Satsangi, R., Pandey, R. et al. In vitro conservation of Bacopa monnieri (L.) using mineral oil. Plant Cell Tiss Organ Cult 111, 291–301 (2012).

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  • Bacopa
  • Bacoside A
  • ‘Brahmi’
  • Genetic stability
  • ISSR
  • Medicinal plant
  • Medium-term conservation
  • Mineral oil overlay
  • RAPD
  • Slow growth