Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 111, Issue 2, pp 209–218 | Cite as

Shoot and root proliferation in ‘Williams’ banana: are the topolins better cytokinins?

  • Adeyemi O. Aremu
  • Michael W. Bairu
  • Lucie Szüčová
  • Karel Doležal
  • Jeffrey F. Finnie
  • Johannes Van Staden
Original Paper

Abstract

The effects of five topolins (meta-Topolin = mT; meta-Topolin riboside = mTR; meta-Methoxy topolin = MemT; meta-Methoxy topolin riboside = MemTR and meta-Methoxy topolin 9-tetrahydropyran-2-yl = MemTTHP) on shoot and root regeneration of micropropagated ‘Williams’ bananas were compared to benzyladenine (BA). Sterile shoot-tip explants were cultured on modified Murashige and Skoog (MS) media containing 10, 20 or 30 μM of the above cytokinins (CKs) for 42 days. Using shoot regenerated from optimum CK concentration, rooting experiments involving the use of indole-3-acetic acid, indole-3-butyric acid, naphthalene acetic acid, smoke–water (SW) and karrikinolide (KAR1) were conducted. Apart from 10 μM BA and 30 μM MemTTHP treatments, the number of shoots produced in all the CK treatments were significantly higher than the control. In addition, 30 μM mT resulted in the highest number of shoots (7.3 ± 1.0). Unlike other CK treatments requiring higher concentrations, optimum mean shoot number per explant rate was attained at the lowest concentration in MemT and MemTTHP (10 μM) treatments. The use of 10 μM MemTTHP had the least root inhibitory effect during the shoot proliferation phase. In terms of abnormality index, mTR-regenerated plantlets were of the best quality across all the CKs tested. In mT- and BA-derived shoots, SW and KAR1 significantly increased the number and length of roots compared to the control. Overall, when compared to BA, the use of topolin demonstrated higher mean shoot number per explant (MemT and MemTTHP) at lowest CK concentrations and the ease of rooting during the shoot proliferation phase (MemTTHP). During the rooting phase, topolin treatments produced more off-shoots than BA-treated ones. The current finding is a further demonstration of the increasing importance of topolins in micropropagation.

Keywords

Auxins Karrikinolide Musa spp. Physiological abnormalities Plant tissue culture Rooting Smoke–water 

Abbreviations

ANOVA

Analysis of variance

BA

N6-benzyladenine

CK

Cytokinin

DMRT

Duncan’s multiple range test

DW

Dry weight

FW

Fresh weight

IAA

Indole-3-acetic acid

IBA

Indole-3-butyric acid

KAR1

Karrikinolide

MemT

meta-Methoxy topolin

MemTR

meta-Methoxy topolin riboside

MemTTHP

meta-Methoxy topolin 9-tetrahydropyran-2-ylpurine

MS

Murashige and Skoog medium

mT

meta-Topolin

mTR

meta-Topolin riboside

NAA

α-Naphthalene acetic acid

PGR

Plant growth regulator

PPF

Photosynthetic photon flux

PTC

Plant tissue culture

SW

Smoke–water

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Adeyemi O. Aremu
    • 1
  • Michael W. Bairu
    • 1
  • Lucie Szüčová
    • 2
    • 3
  • Karel Doležal
    • 2
    • 3
  • Jeffrey F. Finnie
    • 1
  • Johannes Van Staden
    • 1
  1. 1.School of Life Sciences, Research Centre for Plant Growth and DevelopmentUniversity of KwaZulu-Natal PietermaritzburgScottsvilleSouth Africa
  2. 2.Laboratory of Growth RegulatorsInstitute of Experimental Botany AS CR, Palacký UniversityOlomoucCzech Republic
  3. 3.Faculty of Science, Centre of the Region Haná for Biotechnological and Agricultural ResearchPalacký UniversityOlomoucCzech Republic

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