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Biochemical profile of callus cultures of Pogostemon cablin (Blanco) Benth

Abstract

Patchouli is an aromatic shrub of commercial interest because its essential oil is rich in patchoulol. This study aimed to evaluate the effect of growth regulators on callus production, analyze the essential oil production in calli and evaluate metabolic differences between callus, in vitro grown-plantlets and greenhouse-grown plants in three different accessions of patchouli. Calli were induced from leaf explants on media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) in combination with 6-benzyladenine (BA). The largest size calli from different accessions were obtained in the presence of the two plant growth regulators (PGRs). For accession POG014, presence of 0.022 mg l−1 2,4-D plus 0.022 mg l−1 BA were optimum. For accession POG021, presence of 0.110 mg l−1 2,4-D plus 0.022 mg l−1 of BA induced the largest callus, whereas for accession POG002, 0.022 mg l−1 2,4-D and 0.225 mg l−1 BA, as well as 0.11 mg l−1 2,4-D and 0.022 mg l−1 BA promoted the development of largest callus. Among all accessions, peroxidase activity was highest in organogenic calli of accession POG014, whereas, polyphenol oxidase activity was highest in in vitro-grown plantlets of accession POG021. Biochemical variables differed significantly among the treatments, with the exception of total sugar levels. The highest concentrations of total sugars were observed in the calli and in vitro-grown plantlets of POG014 and POG021. Essential oils were not detected in callus tissues.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

BA:

6-benzyladenine

AGB:

Active germoplasm bank

GC–MS:

Gas chromatograph–mass spectrometer

MS:

Murashige and Skoog culture medium

POX:

Peroxidase

PPO:

Polyphenol oxidase

PGRs:

Plant growth regulators

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Acknowledgments

We thank the FAPITEC/SE for funding this research and for granting a Master’s Scholarship to the first author. We thank CNPq for the research productivity grants awarded to the second and third author.

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Correspondence to Roberta Miranda Pereira Fernandes.

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Santos, A.V., de Fátima Arrigoni-Blank, M., Blank, A.F. et al. Biochemical profile of callus cultures of Pogostemon cablin (Blanco) Benth. Plant Cell Tiss Organ Cult 107, 35–43 (2011). https://doi.org/10.1007/s11240-011-9953-3

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  • DOI: https://doi.org/10.1007/s11240-011-9953-3

Keywords

  • Patchouli
  • Indirect organogenesis
  • Peroxidase
  • Polyphenol oxidase
  • Total and reducing sugar
  • Total amino acids