An interspecific somatic hybrid between upland cotton (G. hirsutum L. cv. ZDM-3) and wild diploid cotton (G. klotzschianum A.)

Abstract

Somatic hybrids were produced through protoplast electrofusion between the tetraploid cotton Gossypium hirsutum L. cv. ZDM-3 and the wild diploid cotton G. klotzschianum. Hybrid plants were generated from 3 out of 24 callus lines that were derived from fused protoplasts. Hybrid plants were initially identified as somatic hybrids by ploidy analysis: the plants from the 3 callus lines had chromosome numbers near to sum of the two parents (78 = 52 + 26). The plants from the 3 lines were subsequently confirmed as hybrids by cytological, molecular, histological and morphological analyses. The morphology of hybrids was distinct from that of the parents, with elongated stigmas and malformed anthers lacking microspores and pollen, leading to male sterility. It is expected that the male sterility resulted from the high number of univalent and irregular multivalent chromosome pairings per meiocyte.

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Abbreviations

2, 4-D:

2, 4-dichlorophenoxyacetic acid

NAA:

α-Naphthaleneacetic acid

IBA:

Indole-3-butyric acid

KT:

Kinetin

PCR:

Polymerase chain reaction

RAPD:

Random amplified polymorphic DNA

SSR:

Simple sequence repeats

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Acknowledgments

This study was supported by the National Basic Research Program (973 Program. No: 2004CB117305) and the National Natural Science Foundation of China (No: 30471108 and No: 30700511).

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Correspondence to Shuijin Zhu.

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Sun, Y., Liu, S., Wang, Y. et al. An interspecific somatic hybrid between upland cotton (G. hirsutum L. cv. ZDM-3) and wild diploid cotton (G. klotzschianum A.). Plant Cell Tiss Organ Cult 106, 425–433 (2011). https://doi.org/10.1007/s11240-011-9939-1

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Keywords

  • Cotton
  • Somatic hybrid
  • Protoplast fusion
  • Plant regeneration
  • Male sterility