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Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 106, Issue 3, pp 425–433 | Cite as

An interspecific somatic hybrid between upland cotton (G. hirsutum L. cv. ZDM-3) and wild diploid cotton (G. klotzschianum A.)

  • Yuqiang Sun
  • Shoumei Liu
  • Yu Wang
  • Brian Joseph Jones
  • Huizhong Wang
  • Shuijin ZhuEmail author
Original Paper

Abstract

Somatic hybrids were produced through protoplast electrofusion between the tetraploid cotton Gossypium hirsutum L. cv. ZDM-3 and the wild diploid cotton G. klotzschianum. Hybrid plants were generated from 3 out of 24 callus lines that were derived from fused protoplasts. Hybrid plants were initially identified as somatic hybrids by ploidy analysis: the plants from the 3 callus lines had chromosome numbers near to sum of the two parents (78 = 52 + 26). The plants from the 3 lines were subsequently confirmed as hybrids by cytological, molecular, histological and morphological analyses. The morphology of hybrids was distinct from that of the parents, with elongated stigmas and malformed anthers lacking microspores and pollen, leading to male sterility. It is expected that the male sterility resulted from the high number of univalent and irregular multivalent chromosome pairings per meiocyte.

Keywords

Cotton Somatic hybrid Protoplast fusion Plant regeneration Male sterility 

Abbreviations

2, 4-D

2, 4-dichlorophenoxyacetic acid

NAA

α-Naphthaleneacetic acid

IBA

Indole-3-butyric acid

KT

Kinetin

PCR

Polymerase chain reaction

RAPD

Random amplified polymorphic DNA

SSR

Simple sequence repeats

Notes

Acknowledgments

This study was supported by the National Basic Research Program (973 Program. No: 2004CB117305) and the National Natural Science Foundation of China (No: 30471108 and No: 30700511).

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Yuqiang Sun
    • 1
    • 2
  • Shoumei Liu
    • 1
  • Yu Wang
    • 1
  • Brian Joseph Jones
    • 3
  • Huizhong Wang
    • 1
  • Shuijin Zhu
    • 2
    Email author
  1. 1.College of Life and Environmental ScienceHangzhou Normal UniversityZhejiangChina
  2. 2.College of Agriculture and BiotechnologyZhejiang UniversityHangzhouPeople’s Republic of China
  3. 3.Faculty of Agriculture, Food and Natural ResourcesThe University of SydneySydneyAustralia

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