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Characterization and pharmacological properties of in vitro propagated clones of Echinacea tennesseensis (Beadle) Small

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Abstract

Tissue culture techniques have been used to establish and maintain a repository of medicinal Echinacea. In vitro clones obtained from hypocotyls of germinated seeds, varied macroscopically, microscopically and exhibited variation in immune enhancing activity. Two in vitro produced clones of Echinacea tennesseensis (Beadle) Small (ETN 03 and ETN 11) were identified as high and low activity based on the activation of human monocytes. Phenotypic analyses of ETN 03 and ETN 11 clones were done using AFLP (Amplified Fragment Length Polymorphism) assay. Results of the AFLP assay revealed that no mutation has occurred during in vitro multiplication, storage, and acclimatization into soil. Plants of ETN 03, ETN 11 clones were cultivated for two growing seasons. Extracts of their dry leaves and roots exhibited immune enhancing activity; however, the variation in activity noticed between clones during micropropagation diminished and was no longer statistically relevant.

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Acknowledgments

This research was partially funded by grants from the National Institutes of Health RO1 AT002360 (NCAAM) to DSP and the USDA, Agricultural Research Service Specific Cooperative Agreement No. 58-6408-7-012.

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Authors and Affiliations

  1. National Center for Natural Products Research, School of Pharmacy, The University of Mississippi , University, Oxford, MS, 38677, USA

    Rita M. Moraes, Hemant Lata, Joko Sumyanto, Vaishali C. Joshi, Nirmal D. Pugh, Ikhlas A. Khan & David S. Pasco

  2. Center of Water and Wetland Resources, The University of Mississippi Field Station, 15 Road 2078, Abbeville, MS, 38601, USA

    Rita M. Moraes, Joko Sumyanto & Ikhlas A. Khan

  3. Plant Biotechnology Center, The University of Ribeirao Preto, Av. Costabile Romano 2201, Ribeirao Preto, SP, 14096-380, Brazil

    Ana M. S. Pereira & Bianca W. Bertoni

  4. Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, Oxford, MS, 38677, USA

    Ikhlas A. Khan & David S. Pasco

Authors
  1. Rita M. Moraes
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  2. Hemant Lata
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  3. Joko Sumyanto
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  4. Ana M. S. Pereira
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  5. Bianca W. Bertoni
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  6. Vaishali C. Joshi
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  7. Nirmal D. Pugh
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  8. Ikhlas A. Khan
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  9. David S. Pasco
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Correspondence to Rita M. Moraes.

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Moraes, R.M., Lata, H., Sumyanto, J. et al. Characterization and pharmacological properties of in vitro propagated clones of Echinacea tennesseensis (Beadle) Small. Plant Cell Tiss Organ Cult 106, 309–315 (2011). https://doi.org/10.1007/s11240-011-9922-x

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  • DOI: https://doi.org/10.1007/s11240-011-9922-x

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